Jason Kay
Jason Kay
Associate Professor
Specialty/Research Focus
Microbiology; Oral Biology
Education and Training:
- Postdoctoral Fellow, Hospital for Sick Children (2013)
- Postdoctoral Fellow, University of Queensland (2008)
- PhD, Molecular Cell Biology, University of Queensland (2007)
- MS, Molecular Microbiology, University of Calgary (2000)
- BS, Biochemistry, University of Calgary (1998)
Employment:
- Associate Professor, Oral Biology, University at Buffalo (2020-present)
- Assistant Professor, Oral Biology, University at Buffalo (2013–2019)
Awards and Honors:
- Dean's Commendation (2008)
- Keystone Macrophage conference travel award (2007)
- ASMR Queensland Award for Health and Medical Research (Finalist) (2006)
Research Expertise:
- Cell Biology
- Immunology
- pathogen-host interactions
- Phagocytosis
Grants and Sponsored Research:
- June 2022–May 2027
Lipid mediated oral tolerance
NIH/NIAID
Role: Co-Principal Investigator
$2,327,530 - June 2022–May 2025
Cooperativity of Oral Microbes in Evading Neutrophil-Mediated Killing
NIH/NIDCR
Role: Co-Investigator
$97,365 - December 2019–November 2024
A role for immune cell plasticity in commensal survival and escalation of inflammation
NIH/NIDCR
Role: Principal Investigator
$1,952,242 - September 2019–September 2022
Mechanisms of Oral Streptococcus Macrophage Activation
NIH/NIDCR
Role: Co-Investigator
$93,568 - August 2019–July 2020
Spinning Disk Confocal Microscope
NIH/OD
Role: Principal Investigator
$487,743 - July 2016–June 2018
Mechanisms of Streptococcus gordonii survival within phagocytes.
NIH/NIDCR
Role: Principal Investigator
$239,250
Journal Articles:
- Almarghlani A, Settem RP, Croft AJ, Metcalfe S, Giangreco M, Kay JG. Interleukin-34 permits Porphyromonas gingivalis survival and NF-kB p65 inhibition in macrophages. Mol Oral Microbiol. 2022; 37(3).
- Metcalfe S, Anselmi N, Escobar A, Visser MB, Kay JG. Innate Phagocyte Polarization in the Oral Cavity. Front Immunol. 2022; 12.
- Nguyen NH, Glassman FY, Dingman RK, Shenoy GN, Wohlfert EA, Kay JG, Bankert RB, Balu-Iyer SV. Rational design of a nanoparticle platform for oral prophylactic immunotherapy to prevent immunogenicity of therapeutic proteins. Sci Rep. 2021; 11(1).
- Pradhan AJ, Lu D, Parisi LR, Shen S, Berhane IA, Galster SL, Bynum K, Monje-Galvan V, Gokcumen O, Chemler SR, Qu J, Kay JG, Atilla-Gokcumen GE. Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis. Cell Chem Biol. 2021.
- Salvatori O, Kumar R, Metcalfe S, Vickerman M, Kay JG, Edgerton M. Bacteria Modify Candida albicans Hypha Formation, Microcolony Properties, and Survival within Macrophages. mSphere. 2020; 5(4).
- Kay JG, Fairn GD. Distribution, dynamics and functional roles of phosphatidylserine within the cell. Cell Commun Signal. 2019; 17(1).
- Kay JG, Kramer JG, Visser MB. Danger signals in oral cavity-related diseases. J Leukoc Biol. 2019.
- Salvatori O, Pathirana RU, Kay JG, Edgerton M. Candida albicans Ras1 inactivation increases resistance to phagosomal killing by human neutrophils. Infect Immun. 2018.
- Croft AJ, Metcalfe S, Honma K, Kay JG. Macrophage Polarization Alters Postphagocytosis Survivability of the Commensal Streptococcus gordonii. Infect Immun. 2018; 86(3).
- Pathirana RU, Friedman J, Norris HL, Salvatori O, McCall AD, Kay JG, Edgerton M. Fluconazole-Resistant Candida auris Is Susceptible to Salivary Histatin 5 Killing and to Intrinsic Host Defenses. Antimicrob Agents Chemother. 2018; 62(2).
- Hirama T, Lu SM, Kay JG, Maekawa M, Kozlov MM, Grinstein S, Fairn GD. Membrane curvature induced by proximity of anionic phospholipids can initiate endocytosis. Nat Commun. 2017; 8(1).
- Hirama T, Das R, Yang Y, Ferguson C, Won A, Yip CM, Kay JG, Grinstein S, Parton RG, Fairn GD. Phosphatidylserine dictates the assembly and dynamics of caveolae in the plasma membrane. J Biol Chem. 2017.
- Chinthamani S, Settem RP, Honma K, Kay JG, Sharma A. Macrophage inducible C-type lectin (Mincle) recognizes glycosylated surface (S)-layer of the periodontal pathogen Tannerella forsythia. PLoS One. 2017; 12(3).
- Varanat M, Haase EM, Kay JG, Scannapieco FA. Activation of the TREM-1 Pathway in Human Monocytes by Periodontal Pathogens and Oral Commensal Bacteria. Mol Oral Microbiol. 2017; 32(4).
- Saric A, Hipolito VE, Kay JG, Canton J, Antonescu CN, Botelho RJ. mTOR controls lysosome tubulation and antigen presentation in macrophages and dendritic cells. Mol Bio Cell. 2016; 27(2).
- Kay JG, Grinstein S. Phosphatidylserine-mediated cellular signaling. Adv Exp Med Biol. 2013; 991.
- Mrakovic A, Kay JG, Furuya W, Brumell JH, Botelho RJ. Rab7 and Arl8 GTPases are necessary for lysosome tubulation in macrophages. Traffic. 2012; 13(12).
- Kay JG, Koivusalo M, Ma X, Wohland T, Grinstein S. Phosphatidylserine dynamics in cellular membranes. Mol Biol Cell. 2012; 23(11).
- Golebiewska U, Kay JG, Masters T, Grinstein S, Im W, Pastor RW, Scarlata S, McLaughlin S. Evidence for a fence that impedes the diffusion of phosphatidylinositol 4,5-bisphosphate out of the forming phagosomes of macrophages. Mol Biol Cell. 2011; 22(18).
- Wykes MN, Kay JG, Manderson A, Liu XQ, Brown DL, Richard DJ, Wipasa J, Jiang SH, Jones MK, Janse CJ, Waters AP, Pierce SK, Miller LH, Stow JL, Good MF. Rodent blood-stage Plasmodium survive in dendritic cells that infect naive mice. Proc Natl Acad Sci U S A. 2011; 108(27).
- Kay JG, Grinstein S. Sensing phosphatidylserine in cellular membranes. Sensors (Basel). 2011; 11(2).
- Reefman E, Kay JG, Wood SM, Offenhäuser C, Brown DL, Roy S, Stanley AC, Low PC, Manderson AP, Stow JL. Cytokine secretion is distinct from secretion of cytotoxic granules in NK cells. J Immunol. 2010; 184(9).
- Kockx M, Guo DL, Traini M, Gaus K, Kay JG, Wimmer-Kleikamp S, Rentero C, Burnett JR, Le Goff W, Van Eck M, Stow JL, Jessup W, Kritharides L. Cyclosporin A decreases apolipoprotein E secretion from human macrophages via a protein phosphatase 2B-dependent and ATP-binding cassette transporter A1 (ABCA1)-independent pathway. J Biol Chem. 2009; 284(36).
- Desclozeaux M, Venturato J, Wylie FG, Kay JG, Joseph SR, Le HT, Stow JL. Active Rab11 and functional recycling endosome are required for E-cadherin trafficking and lumen formation during epithelial morphogenesis. Am J Physiol Cell Physiol. 2008; 295(2).
- Huynh KK, Kay JG, Stow JL, Grinstein S. Fusion, fission, and secretion during phagocytosis. Physiology (Bethesda). 2007; 22.
- Kockx M, Guo DL, Huby T, Lesnik P, Kay JG, Sabaretnam T, Jary E, Hill M, Gaus K, Chapman J, Stow JL, Jessup W, Kritharides L. Secretion of apolipoprotein E from macrophages occurs via a protein kinase A and calcium-dependent pathway along the microtubule network Circ Res. 2007; 101(6).
- Manderson AP, Kay JG, Hammond LA, Brown DL, Stow JL. Subcompartments of the macrophage recycling endosome direct the differential secretion of IL-6 and TNFalpha. J Cell Biol. 2007; 178(1).
- Beck KE, Kay JG, Braun JE. Rdj2, a J protein family member, interacts with cellular prion PrP(C). Biochem Biophys Res Commun. 2006; 346(3).
- Kay JG, Murray RZ, Pagan JK, Stow JL. Cytokine secretion via cholesterol-rich lipid raft-associated SNAREs at the phagocytic cup. J Biol Chem. 2006; 281(17).
- Murray RZ, Kay JG, Sangermani DG, Stow JL. A role for the phagosome in cytokine secretion. Science. 2005; 310(5753).
- Miller LC, Swayne LA, Kay JG, Feng ZP, Jarvis SE, Zamponi GW, Braun JE. Molecular determinants of cysteine string protein modulation of N-type calcium channels. J Cell Sci. 2003; 116(Pt 14).
- Swayne LA, Blattler C, Kay JG, Braun JE. Oligomerization characteristics of cysteine string protein. Biochem Biophys Res Commun. 2003; 300(4).
- Greene EA, Kay JG, Stehmeier LG, Voordouw G. Microbial community composition at an ethane pyrolysis plant site at different hydrocarbon inputs. FEMS Microbiol Ecol. 2002; 40(3).
- Magga JM, Kay JG, Davy A, Poulton NP, Robbins SM, Braun JE. ATP dependence of the SNARE/caveolin 1 interaction in the hippocampus. Biochem Biophys Res Commun. 2002; 291(5).
- Greene EA, Kay JG, Jaber K, Stehmeier LG, Voordouw G, Kay J. Composition of soil microbial communities enriched on a mixture of aromatic hydrocarbons. Appl Environ Microbiol. 2000; 66(12).
Professional Memberships:
- American Society for Microbiology (2017–present)
- Society for Leukocyte Biology (2014–present)
- American Association for Dental Research (2013–present)
- International Association for Dental Research (2013–present)
- American Society for Cell Biology (2008–present)
Presentations:
- "Macrophage-Commensal Dynamics Under Inflammatory Activation." International Association for Dental Research Annual Meeting, International Association for Dental Research (2019)
- "IL-34 Alters Macrophage Killing Ability" Buffalo Conference on Microbial Pathogenesis, Witebsky Center, University at Buffalo (2019)
- "Macrophage-Commensal Dynamics Under Inflammatory Activation." Buffalo Conference on Microbial Pathogenesis, Witebsky Center, University at Buffalo (2019)
- "Candida albicans Pathogenic Phenotypes are Modified by Bacterial Interactions." SDM annual Research Day, School of Dental Medicine, University at Buffalo (2019)
- "Macrophage-Commensal Dynamics During Inflammation." SDM annual Research Day, School of Dental Medicine, University at Buffalo (2019)
- "Inflammatory Activation Alters Macrophage-Commensal Dynamics." Society for Leukocyte Biology Annual Meeting, Society for Leukocyte Biology (2018)
- "An Investigation of Commensal-Immune Interactions Altered by Oral Pathogens." SUNY Genesco undergraduate research day, SUNY Genesco (2018)
- "Inflammatory Activation Alters Macrophage-Commensal Dynamics" Annual Buffalo Conference on Microbial Pathogenesis,, University at Buffalo, Witebsky Center for Microbial Pathogenesis and Immunology (2018)
- "Candida albicans RAS1 modulates survival within human neutrophils" American Association for Dental Research Annual Meeting, American Association for Dental Research (2018)
- "Imaging phagosomal survival of commensal oral microbes." 20th Annual Upstate New York Immunology Conference, Upstate New York Immunology (2017)
- "Ratiometric Imaging to Measure Phagosome Escape of Oral Microbes" 29th Annual Buffalo Conference on Microbial Pathogenesis, The Witebsky Center for Microbial Pathogenesis and Immunology (2017)
- "Candida albicans stress response MAPKinases protect from Phagocytic Killing" American Association for Dental Research Annual Meeting, American Association for Dental Research (2017)
- "Determining the Fate of Phagocytosed Porphyromonas gingivalis" American Association for Dental Research Annual Meeting, American Association for Dental Research (2017)
- "Changing landscapes: oral commensal survival within phagocytes" Mark Wilson Conference, Oral Immunology/Microbiology Research group (2017)
- "When good bugs go bad: Oral Microbes Resisting phagocytic Destruction" Seminar series, University at Buffalo, Microbiology and Immunology (2017)
- "Determining the Fate of Phagocytosed Porphyromonas gingivalis" Annual Research Day, University at Buffalo, School of Dental Medicine (2017)
- "Determining the Fate of Phagocytosed Porphyromonas gingivalis." American Association of Women Dentists Annual Meeting, American Association of Women Dentists (2017)
- "Effects of IL-34 vs M-CSF maturation on macrophage interactions with Porphyromonas gingivalis" Annual Research Day, University at Buffalo, School of Dental Medicine (2017)
- "Ratiometric Imaging to Measure Phagosome Escape of Oral Microbes" 2nd Annual Immune Imaging Symposium, University of Rochester, Program for Advanced Immune Bioimaging (2016)
- "Avoidance of Phagocytic Destruction by Strptococcus gordonii" 28th Annual Buffalo Conference on Microbial Pathogenesis, University at Buffalo, The Witebsky Center for Microbial Pathogenesis and Immunology (2016)
- "Sorting Nexins in Phagosomal Maturation" 2015-16 Biology honors program presentations, University at Buffalo, Department of Biology (2016)
- "Avoidance of Phagocytic Destruction by Streptococcus gordonii" 2016 General Session, American Association for Dental Research (2016)
- "Factors Affecting Candida albicans Phagocytosis and Killing by Phagocytic Cells" 2016 General Session, American Association for Dental Research (2016)
- "AbpA may protect Streptococcus gordonii from oxidative stress" Student Research Day, University at Buffalo, School of Dental Medicine (2016)
- "Determining the Fate of Internalized Porphyromonas gingivalis within Phagocytic Cells" Student Research Day, University at Buffalo, School of Dental Medicine (2016)
- "Understanding the relationship between Rab 5 GTPase and protein Sorting Nexin 8 in phagocytosis" Annual poster session, University at Buffalo, CLIMB UP for Summer Research at University at Buffalo (2015)
- "Determining the Fate of Internalized Porphyromonas gingivalis within Phagocytic Cells" 2015 Summer Biomedical Student Research Day, University at Buffalo (2015)
- "Sorting Nexin Proteins Involvement in Phagocytosis and Effects on Phagosome Maturation" 2014-15 Biology honors program presentations, University at Buffalo, Department of Biology (2015)
- "Evasion of Phagosomal Destruction by S. gordonii" 2015 Buffalo Immunology Conference, The Witebsky Center for Microbial Pathogenesis and Immunology (2015)
- "Cholesterol dictates the subcellular distribution of phosphatidylserine" Lysosomes and Endocytosis, Gordon Research Conferences (2014)
- "mTOR is Required for Lysosome Tubulation and MHCII Trafficking in Macrophages and Dendritic Cells" Lysosomes and Endocytosis, Gordon Research Conferences (2014)
- "Recruitment of Sorting Nexin to the Maturing Phagosome" 2013-14 Biology honors program presentations, University at Buffalo, Department of Biology (2014)
- "Role of lipids and lysosomes in innate immunity; insights from advanced live imaging of macrophages" Invited Speaker, University of Louiseville, Center for Oral Health and Systemic Disease (2013)
- "Role of lipids and lysosomes in innate immunity; insights from advanced live imaging of macrophages" Invited speaker, University of Alberta, School of Dentistry (2012)
- "Role of lipids and lysosomes in innate immunity; insights from advanced live imaging of macrophages" Invited speaker, University at Buffalo, Department of Oral Biology (2012)
- "Characterization of Phosphatidylserine Dynamics in Membranes of Live Cells" Gordon Research Conference - Lysosomes and Endocytosis, Gordon Research Conferences, Lysosomes and Endocytosis (2012)
- "The Role of Arl8b and Rab7 GTPases in Lysosome Tubulation" American Society for Cell Biology Annual Meeting (2011)
- "Phosphatidylserine Dynamics in Cellular Membranes" Gordon Research Conference - Molecular and Cellular Biology of Lipids, Molecular and Cellular Biology of Lipids (2011)
- "A Diffusional Barrier in the Phagocytic Cup" American Society for Cell Biology Annual Meeting, American Society for Cell Biology (2009)
- "Dual Roles for the Recycling Endosome in Phagocytosis" Gordon Research Conference - Lysosomes and Endocytosis, Lysosomes and Endocytosis (2008)
- "Imaging Cytokine Secretion in Macrophages: Imaging to demonstrate subdomains of the recycling endosome" Hunter Cellular Biology Meeting (2008)
- "Protein Kinase A in the Regulation of Macrophage Apolipotprotein E Trafficking and Secretion" Hunter Cellular Biology Meeting (2008)
- "Modification of the Phagocytic Cup by Lipid Rafts" Keystone Macrophage conference (2007)
- "Differential Requirement for Recycling Endosome in Phagocytosis and Cytokine Secretion" Australasian Society for Immunology 37th Annual Scientific Meeting (2007)
- "Cytokine Secretion, Phagocytosis and Cholesterol" Queensland Premier Awards for Health and Medical Research (2006)
- "Lipid Raft Dependence of Cytokine Secretion and Phagocytosis in Macrophages" Australian Society for Biochemistry and Molecular Biology ComBio (2006)
- "Caveolin in the Hippocampus and its Interaction with SNAREs" Society for Neuroscience 32nd Annual Meeting, Society for Neuroscience (2002)
- "Distinct modes of action by the J domain and the cysteine string domain of CSP modulate G protein function" Society for Neuroscience 32nd annual Meeting (2002)
- "Molecular basis of the modulation of calcium channel function by cysteine string protein" Society for Neuroscience 32nd annual Meeting (2002)
- "Caveolin in the Hippocampus and its Interaction with SNAREs" Alberta Neuroscience Conference (2002)
- "Biodegradation of C5+ Hydrocarbon Compounds by Aerobic and Anaerobic Communities Enriched from Pyrolysis Plant Soils" Canadian Society of Microbiology Annual Meeting (2000)
- "Soil Microbial Community Responses to Incubation in C5+ Followed by DCPD" Canadian Society of Microbiology Annual Meeting (2000)
- "Characterisation of C5+ Degrading Microbial Communities from Soil" Canadian Society of Microbiology Annual Meeting (1999)
Service Activities:
- School of Dental Medicine Admissions Committee; Member (2015)
- Oral Biology, School of Dental Medicine Graduate Committee, D.D.S./Ph.D.; Member (2015)
- School of Dental Medicine Ad Hoc Committee; Member (2015)
- Oral Biology, School of Dental Medicine Graduate Committee, Preliminary Exams; Member (2015)
- Oral Biology, School of Dental Medicine Graduate Committee, Preliminary Exams; Member (2015)
- Oral Biology, School of Dental Medicine Graduate Committee, D.D.S./Ph.D.; Member (2014)
- School of Dental Medicine Student Research and Honors Committee; Member (2014)
- Oral Biology, School of Dental Medicine Graduate Committee, Preliminary Exams; Member (2013–2014)
- Core Director Search committee; Search committee to find core director for new SDM-managed Confocal microscopy core; Chair (2018)
- AADR Buffalo Section; President (2016–present)
- AADR Buffalo Section; Treasurer (2015–2016)
- Society for Leukocyte Biology - Membership committee; Member (2015–present)
- School of Dental Medicine Admissions Committee; Member (2015–present)
- External Thesis Examiner; Thesis examiner for Xiaying Qi, University of Queensland; Examiner (2015)
- Student Research and Honors Committee; Member (2014–present)
- Oral Biology Seminar Series; Coordinator (2014–present)
- AADR Buffalo Section; Secretary (2014–2015)