Elaine M. Haase
Elaine M. Haase
Research Professor
Specialty/Research Focus
Microbiology; Oral Biology
Professional Summary:
I began my career as a general Medical Technologist and eventually specialized in clinical bacteriology in local hospital. I returned to school to earn an MS in Medical Technology specializing in Microbiology. I continued to work weekends in the hospital while working as a research technician in immunodermatology. I pursued further graduate work and earned a PhD degree in Microbiology from UB. During my thesis work I became interested in molecular mechanisms of infectious disease. As a post-doc I focused on characterizing the genes of the fimbrial operon and biofilm formation by the periodontopathogen Aggregatibacter actinomycetemcomitans. These studies expanded into gene regulation in A. actinomycetemcomitans by small non-coding sRNA. We have recently used RNA-Seq to investigate the effect of amylase on Streptococcus gordonii. Whole genome sequencing has been used to explore the phylogeny of amylase-binding proteins in various Streptococcal strains. We are also discovering potential new functions for amylase-binding protein A from Streptococcus gordonii and pursuing new experimental evidence. We are also working on oral microbiome projects with clinical physicians and dentists.
Education and Training:
- PhD, Microbiology, University at Buffalo (1995)
- MS, Medical Technology, Microbiology Specialty, University at Buffalo (1984)
- BS, Medical Technology, University at Buffalo (1975)
Employment:
- Research Professor, University at Buffalo (2018-present)
- Research Associate Professor, Oral Biology, State University of New York at Buffalo (2005–2017)
- Research Assistant Professor, Oral Biology, State University of New York at Buffalo (1998–2005)
- Post-Doctoral Fellow, Oral Biology, State University of New York at Buffalo (1994–1998)
- Research Assistant, Oral Biology, State University of New York at Buffalo (1994)
- Research Assistant, Microbiology and Immunology, State University of New York at Buffalo (1986–1994)
- Medical Technologist, St. Mary‘s Hospital (1975–1991)
- Research Technician, Microbiology and Immunology, State University of New York at Buffalo (1985–1986)
Awards and Honors:
- Dental Student Research Mentor Award (2016)
- Listed in Who's Who in America (2010)
- American Society for Microbiology Student Travel Award (1994)
- Listed in Who's Who Among Students in American Universities and Colleges (1992)
- American Society for Microbiology Student Travel Award (1990)
- Erwin Neter Award from WNY American Society of Microbiology (1990)
Research Expertise:
- Bioinformatics: Analysis of RNA-seq and DNA-seq data.
- Microbiologist: Bacterial adhesion mechanisms, biofilm studies, protein analysis, regulation of biofilms, and molecular biology. Bioinformatic and microbiome studies.
Grants and Sponsored Research:
- April 2012–April 2017
Amylase Binding and Gene Expression in S. gordonii.
NIH-NIDCR
Role: Co-Investigator
$1,250,000 - April 2004–December 2008
Amylase-Binding Streptococci, Dental Plaque and Caries
NIH, USPHS
Role: Co-Investigator
$1,532,810 - July 2004–June 2007
Oral Health and Ventilator-Associated Pneumoniae
NIH, USPHS
Role: Co-Investigator
$1,140,607 - July 2004–January 2007
New Salicylanilides to Treat Oral Diseases
NIH/SBIR
Role: Co-Investigator - January 2002–December 2003
Studies of the flp operon in A. actinomycetemcomitans
National Institute for Dental & Craniofacial Research
Role: Principal Investigator
$156,000
Journal Articles:
- Janem, Waleed F., Scannapieco FA, Sabharwal A, Tsompana, Maria, Berman, Harvey A.,Haase EM, Miecznikowski, Jeffrey C., Mastrandrea, Lucy D.. Salivary Inflammatory Markers and Microbiome in Normoglycemic Lean and Obese Children Compared to Obese Children with Type 2 Diabetes.. PLOS One. 2017.
- Mashima I, Djais AA, Haase EM, Scannapieco FA, Otomo M, Saitoh M, Nakazawa F. Establishment of a Species-specific Primer Pair for Detecting Veillonella infantium Based on the 70-kDa Heat Shock Protein Gene dnaK. Anaerobe. 2018; 52.
- Mashima I, Liao YC, Sabharwal A, Haase EM, Nakazawa F, Scannapieco FA. Draft Genome Sequences of Four Strains of Recently Established Novel Veillonella species Isolated from Human Oral Cavities. Genome Announc. 2018; 6(15).
- Haase EM, Kou Y, Sabharwal A, Liao YC, Lan T, Lindqvist C, Scannapieco FA. Comparative Genomics and Evolution of the Amylase-binding Proteins of Oral Streptococci. BMC Microbiol. 2017; 17(1).
- Varanat M, Haase EM, Kay JG, Scannapieco FA. Activation of the TREM-1 Pathway in Human Monocytes by Periodontal Pathogens and Oral Commensal Bacteria. Mol Oral Microbiol. 2017; 32(4).
- Liang,X; Liu,B; Zhu,F; Scannapieco,FA; Haase,EM; Matthews,S; Wu,Hui. A Distinct Sortase SrtB Anchors and Processes a Streptococcal Adhesin AbpA with a Novel Structural Property.. Sci Rep. 2016.
- Liao YC, Lin HH, Sabharwal A, Haase EM, Scannapieco FA. MyPro: A Seamless Pipeline for Automated Prokaryotic Genome Assembly and Annotation. J Microbiol Methods. 2015; 113.
- Haase EM, Feng X, Pan J, Miecznikowski JC, Scannapieco FA. Dynamics of the Streptococcus gordonii Transcriptome in Response to Media Composition, Salivary a-amylase and Starch. Appl Environ Microbiol. 2015.
- Sabharwal A, Liao YC, Lin HH, Haase EM, Scannapieco FA. Draft Genome Sequences of 18 Oral Streptococcus Strains that encode Amylase-binding Proteins. Genome Announc. 2015; 3(3).
- Maddi A, Haase E, Scannapieco F, Haase EM. Mass Spectrometric Analysis of Whole Secretome and Amylase-precipitated Secretome Proteins from Streptococcus gordonii. J Proteomics Bioinform. 2014; 7.
- Li Q, Pan C, Teng D, Lin L, Kou Y, Haase EM, Scannapieco FA, Pan Y. Porphyromonas gingivalis Modulates Pseudomonas aeruginosa-induced Apoptosis of Respiratory Epithelial Cells Through the STAT3 Signaling Pathway. Microbes Infect. 2014; 16(1).
- Nikitkova AE, Haase EM, Scannapieco FA. Taking the Starch Out of Oral Biofilm Formation: Molecular Basis and Functional Significance of Salivary a-amylase Binding to Oral Streptococci. Appl Environ Microbiol. 2013; 79(2).
- Violante TL, Haase EM, Vickerman MM. Collagen-binding Streptococcal Surface Proteins Influence the Susceptibility of Biofilm Cells to Endodontic Antimicrobial Solutions. J Endod. 2013; 39(3).
- Heo SM, Choi KS, Kazim LA, Reddy MS, Haase EM, Scannapieco FA, Ruhl S. Host Defense Proteins derived from Human Saliva Bind to Staphylococcus aureus.. Infect Immun. 2013; 81.
- Amarasinghe JJ, Connell TD, Scannapieco FA, Haase EM. Novel Iron-regulated and Fur-regulated Small Regulatory RNAs in Aggregatibacter actinomycetemcomitans. Mol Oral Microbiol. 2012; 27(5).
- Tanzer JM, Thompson A, Sharma K, Vickerman MM, Haase EM, Scannapieco FA. Streptococcus mutans Out-competes Streptococcus gordonii in vivo. J Dent Res. 2012; 91(5).
- Nikitkova AE, Haase EM, Vickerman MM, Gill SR, Scannapieco FA. Response of Fatty Acid Synthesis Genes to the Binding of Human Salivary Amylase by Streptococcus gordonii. Appl Environ Microbiol. 2012; 78(6).
- Nikitkova A, Haase EM, Scannapieco FA. Effect of Starch and Amylase on the Expression of Amylase-binding Protein A in Streptococcus gordonii.. Mol Oral Microbiol. 2012.
- Bank,TL, Dosen A, Giese RF, Haase EM, and Sojar HT. Atomic Force Spectroscopy Evidence of Non-specific Adhesion of Aggregatibacter actinomycetemcomitans.. J Nanoscience Nanotechnol. 2011; 11.
- Heo SM, Sung RS, Scannapieco FA, and Haase EM. Genetic Relationships Between Candida albicans Strains Isolated from Dental Plaque, Trachea, and Bronchoalveolar Lavage Fluid from Mechanically Ventilated Intensive Care Patients.. J Oral Microbiol. 2011; 3.
- Vorrasi, J.B., Chaudhuri, B., Haase, E.M., and Scannapieco, F.A.. Identification and Characterization of Amylase-Binding Protein C from the Streptococcus mitis NS51.. Mol Oral Microbiol. 2010.
- Amarasinghe JJ, Scannapieco FA, Haase EM. Transcriptional and Translational Analysis of Biofilm Determinants of Aggregatibacter actinomycetemcomitans in Response to Environmental Perturbation. Infect Immun. 2009; 77.
- Pan Y, Teng D, Burke AC, Haase EM, Scannapieco FA. Oral Bacteria Modulate Invasion and Induction of Apoptosis in HEp-2 Cells by Pseudomonas aeruginosa.. Microb Pathog. 2009; 46.
- Chaudhuri B, Paju S, Haase EM, Vickerman MM, Tanzer JM, Scannapieco FA. Amylase-binding Protein B of Streptococcus gordonii is an Extracellular Dipeptidyl-peptidase. Infect Immun. 2008; 76(10).
- Heo S-M, Haase EM, Lesse A, Gill SR, Scannapieco FA. Genetic Relationships Between Respiratory Pathogens Isolated from Dental Plaque and Bronchoalveolar Lavage Fluid from Mechanically Ventilated Intensive Care Patients.. Clin Infect Dis. 2008.
- Haase EM, Scannapieco F, Bonstein T, Palmer RJ Jr. Environmental Influences on Actinobacillus actinomycetemcomitans Biofilm Formation.. Arch Oral Biol. 2006; 51.
- Haraokopakis E, Haase EM, Scannapieco F, Albzreh H, Hajishengallis G. Inhibition of Proinflammatory Activities of Major Periodontal Pathogens by Aqueous Extracts from Elder Flower (Sambucus nigra).. J Periodontol. 2006; 77.
- Bernstein JM, Haase EM, Dryja D, Wolf J, Briles D, Wilding G. Bacterial Interference of Penicillin-Sensitive and Penicillin-Resistant Streptococcus pneumoniae by Streptococcus oralis in an Adenoid Organ Culture: Implications for the Treatment of Recurrent Upper Respiratory Tract Infections in Children and Adults.. Ann Otol Rhinol Lyarngol. 2006; 115.
- Tanzer JM, Grant L, Thompson A, Li L, Rogers JD, Haase EM, Scannapieco FA. Amylase-Binding Protein A (AbpA) and B (AbpB) Differentially Affect Colonization of Rats‘ Teeth by Streptococcus gordonii.. Microbiol. 2003; 149.
- Paju Susanna, Bernstein JM, Haase EM, Scannapieco FA. Molecular Analysis of Bacterial Flora Associated with Chronically Inflamed Maxillary Sinuses.. J Med Microbiol. 2003; 52.
- Haase EM, Stream JO, Scannapieco FA. Transcriptional Analysis of the 5‘-Terminus of the flp Fimbrial Gene Cluster from Actinobacillus actinomycetemcomitans.. Microbiol. 2003; 149.
- Tanzer JM, Baranowski LK, Scannapieco FA, Rogers JD, Haase EM. Oral Colonization and Cariogenicity of Streptococcus gordonii in Specific Pathogen-free TAN:SPFOM(OM)BR Rats Consuming Starch or Sucrose Diet.. Arch Oral Biol. 2000; 46.
- Brown AE, Rogers JD, Haase EM, Zelasko PM, Scannapieco FA. Prevalence of the Amylase-Binding Protein A Gene (abpA) in Oral Streptococci.. J Clin Microbiol. 1999; 37.
- Haase EM, Zmuda JL, Scannapieco FA. Identification and Molecular Analysis of Rough-Colony-Specific Outer Membrane Proteins of Actinobacillus actinomycetemcomitans.. Infect Immun. 1999; 67.
- Rogers JD, Haase EM, Brown AE, Douglas CWI, Gwynn JP, Scannapieco FA. Identification and Analysis of a Gene (abpA) Essential for Amylase Binding to Streptococcus gordonii.. Microbiol. 1998; 144.
- Haase EM, Yi K, Morse GD, Murphy TF. Mapping of Bactericidal Epitopes on the P2 Porin Protein of Nontypeable Haemophilus influenzae.. Infect Immun. 1994; 62.
- Haase EM, Campagnari AA, Sarwar J, Shero M, Wirth M, Cumming CU, Murphy TF. Strain-Specific and Immunodominant Surface Epitopes of the P2 Porin Protein of Nontypeable Haemophilus influenzae.. Infect Immun. 1991; 59.
- Klingman KL, Jansen(Haase) EM, Murphy TF. Nearest Neighbor Analysis of Outer Membrane Proteins of Nontypeable Haemophilus influenzae.. Infect Immun. 1988; 56.
- Haase EM, Deng JS, Beutner EH, Kumar V, Chorzelski TP. Comparison of Commercial Kits for the Detection of Anti-nDNA Antibodies Using Crithidia luciliae.. Amer J Clin Path. 1987; 87.
Abstracts:
- Heo SM, Choi K-S, Kazim AL, Haase EM, Reddy MS, Scannapieco FA, Ruhl S. Host Defense Proteins Derived from Human Saliva Bind to Staphylococcus aureus.. Adv Dent Res. 2014; 26.
- Eidt A, Lee DH, Heo SM, Haase EM, Reischl U, Cisar JO, Scannapieco FA, Ruhl S. Coadhesion Partners for Helicobacter pylori Among Oral Biofilm Microbiota. J Dent Res. 2010; 89.
- Amarasinghe JJ, Scannapieco FA, Haase EM. Novel Iron-Regulated sRNAs Involved in Biofilm Formation of Aggregatibacter actinomycetemcomitans.. J Dent Res. 2008; 87.
- Heo S-M, Haase EM, Scannapieco FA. Genetic Relationship Between Respiratory Pathogens Isolated from the Mouth and Lung.. J Dent Res. 2008; 87.
- Haase EM, Dunn J, Evans R, Scannapieco FA. Evaluation of Antimicrobial Efficacy Against Biofilms Using the Calgary Biofilm Device.. 4th ASM Conference on Biofilms. 2007.
- Heo S-M, Haase EM, Scannapieco F. Molecular Epidemiology of Plaque and Lung Bacteria from Ventilated Patients.. J Dent Res. 2007; 86.
- Haase EM, Legler R, Bonstein T, Bush PJ, Scannapieco FA. An in vitro Model Assessing Tooth Whitening Agent Effectiveness.. J Dent Res. 2004; 83.
- Paju S, Haase EM, Tanzer JM, Scannapieco FA. Prevalence of the Gene Encoding Amylase-Binding Protein B, a Putative Peptidase, in Oral Streptococci.. J Dent Res. 2003; 82.
- Haase EM, Palmer RJ Jr, Wang Y, Chen C, Kolenbrander P, Scannapieco FA. Localization of the rough colony protein A (RcpA) of Actinobacillus actinomycetemcomitans by immunofluorescence.. J Dent Res. 2002; 81.
- Paju S, Bernstein JM, Haase EM, Scannapieco FA. Non-Cultivable Oral Bacterial Flora of Chronically Inflamed Maxillary Sinuses.. J Dent Res. 2002; 81.
- Haase EM, Scannapieco FA. RNA Analysis of Rough-Colony-Specific Outer Membrane Proteins of Actinobacillus actinomycetemcomitans.. J Dent Res. 2000; 79.
- Haase EM, Zmuda JL, Scannapieco FA. Identification of Rough-Colony-Phenotype Specific Proteins of Actinobacillus actinomycetemcomitans.. J Dent Res. 1999; 78.
- Tanzer JM, Baranowski LK, Scannapieco FA, Rogers J, Haase EM. Streptococcus gordonii Colonizes TAN:SPFOM(OM)BR Rats and is Cariogenic with Sucrose.. J Dent Res. 1998; 77.
- Sanjun IS, Steinhorn DM, Haase EM, Scannapieco FA. Perfluorocarbons Decrease Bacterial Adhesion to Epithelial Cells.. Ped Res. 1998; 43(S2).
- Haase EM, Scannapieco FA. Molecular Cloning of a Putative Amylase-Binding Component from Streptococcus gordonii G9B.. J Dent Res. 1996; 75.
- Rogers JD, Cranston B, Haase EM, Scannapieco FA. Characterization of an Amylase-Binding Deficient Mutant of Streptococcus gordonii Challis.. J Dent Res. 1996; 75.
Professional Memberships:
- Western New York Branch of the American Association for Dental Research; Local branch of the national AADR whose mission is to provide continuing education to those interested in all branches of dental research. (2014–present)
- American Dental Education Association; Professional Membership (2006)
- International/American Association for Dental Research; Professional Membership. (1996–2006)
- Western New York Branch of the American Society for Microbiology; Local branch of the national American Society for Microbiology whose mission is to provide continuing education for those interested in all aspects of microbiology. (1988–present)
- American Society for Microbiology; Professional Membership (1988–present)
- American Society for Clinical Pathologists (1975–2001)
Presentations:
- "The oral microbiome in sickness and i health" Annual Western New York American Society of Microbiology Meeting, American Society of Microbiology, Western New York Branch (2018)
- "Dental Plaque" Student Outreach, Zeptometrix, West Seneca Academy High School Students (2018)
- "Dental Plaque" Student Outreach, Zeptometrix, West Seneca Academy High School Students (2017)
- "So what is so interesting about amylase-binding Streptococci?" WNY AADR, AADR (2015)
- "Transcriptional changes of Streptococcus gordonii amylase-binding protein (abpA) mutant in TSBY medium" Gordon Conference, Salve Regina University, Microbial Adhesion and Signal Transduction (2013)
- "Studies of the Flp Locus of Actinobacillus actinomycetemcomitans" Seminar Series, University of Southern California, Periodontology (2003)
Service Activities:
- American Society for Microbiology; The Committee on Microbial Sciences (COMS) is a program planning committee for the National ASM for the annual general meeting MICROBE.; Committee Member (2019–present)
- Search committee for core facility manager; Committee Member (2018)
- St. Christopher Church, Religious Education; Office assistant for religious education classes. Assist in attendance, answering phone, and other needed tasks. Volunteer at annual Summerfest celebration.; Volunteer (2002–2004)
- Western New York American Society for Microbiology; Current member of the WNY ASM executive committee. Served as Secretary-Treasurer for 3 years, president-elect 1 year, president 1 year, past president for 3 years.; Committee Member (2000–present)
- Laboratory Assistant; Laboratory Assistant for the summer program for Ernest Witebsky Center for Immunology.; Volunteer (1988)