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PubMed Central, Figure 3: Cell Death Differ. 2016 June; 23(6): 1073–1085. Published online 2016 January 15. doi: 10.1038/cdd.2015.162
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Published online 2016 January 15. doi: 10.1038/cdd.2015.162

Figure 3

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Specific induction of Th2 cytokines and chemokines and IL-31 signaling in transgenic skin. (a) Quantitative RT-PCR analysis of expression levels of genes known to promote or be induced by Th2 responses in adult control and transgenic mouse skin. Values were normalized to the housekeeping gene GAPDH. Data are represented as the mean±S.E. of three independent experiments. *P<0.001, **P<0.002, ***P<0.005, Student's t-test. (b) Quantitative RT-PCR analysis of expression levels of genes known to promote Th1 and Th17 responses in adult control and transgenic mouse skin. Data are represented as the mean±S.E. of three independent experiments. (c) Immunofluorescence staining of dorsal skin sections using anti-K5 and IL-33 antibodies. Dotted white lines indicate the epidermal-dermal junction. (d) Quantitative RT-PCR analysis of genes involved in IL-31 signaling in adult transgenic and wild-type mouse skin. Values were normalized to the housekeeping gene GAPDH. Data are represented as the mean±S.E. of three independent experiments. ***P<0.001; **P<0.002, *P<0.005, Student's t-test. Scale bar: 75 μm

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