University at Buffalo - The State University of New York
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 Primer designing tool
PCR Template
(A refseq record is preferred) [?] Clear

From To
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Primer Parameters
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[?] Clear
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Exon/intron selection
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Note: Parameter values that differ from the default are highlighted in yellow
Primer Pair Specificity Checking Parameters
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Clear

Or, upload file:
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Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type. [?]

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Primer must have at least total mismatches to unintended targets, including
at least mismatches within the last bps at the 3' end. [?]
Ignore targets that have or more mismatches to the primer. [?]
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Get Primer
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Advanced parameters Note: Parameter values that differ from the default are highlighted in yellow