University at Buffalo - The State University of New York
Skip to Content
21148692[PMID] - PMC - NCBI

Search results

Items: 3

1.
FIG. 2.

FIG. 2. From: Integrity of Proteins in Human Saliva after Sterilization by Gamma Irradiation .

Effects of irradiation or filtration of whole saliva on the profile and integrity of protein bands after SDS-PAGE. Salivary samples were irradiated with doses up to 11.5 kGy (A) or filtrated in successive steps with membrane filters of decreasing pore size (B). Gels (two different donors) were stained with silver. Molecular masses of protein subunits were estimated by comparison to standard proteins (S).

Stefan Ruhl, et al. Appl Environ Microbiol. 2011 Feb;77(3):749-755.
2.
FIG. 3.

FIG. 3. From: Integrity of Proteins in Human Saliva after Sterilization by Gamma Irradiation .

Effects of irradiation or filtration of whole saliva on the concentration of proteins (A and B) and integrity of fluorophores (C and D), as well as enzymatic activities of amylase (E and F) or lysozyme (G and H). Salivary samples were irradiated (A, C, E, and G) with doses up to 10 kGy or filtrated (B, D, F, and H) with membrane filters of decreasing pore size. Irradiation was performed in three independent experiments on samples from three individuals (•, ▴, and ▪). Each data point represents the result from one donor in one experiment. Filtration was performed in five (B) or two (D, F, and H) independent experiments on samples from two individuals (• and ▴). The dashed horizontal line shows the 50% margin.

Stefan Ruhl, et al. Appl Environ Microbiol. 2011 Feb;77(3):749-755.
3.
FIG. 1.

FIG. 1. From: Integrity of Proteins in Human Saliva after Sterilization by Gamma Irradiation .

Effects of irradiation or filtration on bacterial viability. (A) Suspensions of Escherichia coli, Geobacillus stearothermophilus, and Deinococcus radiodurans were irradiated with doses up to 10 kGy. Irradiation was performed in three independent experiments on pure bacterial suspensions in saline solution (saline data for D. radiodurans) or on salivary samples from three individuals spiked with D. radiodurans (saliva data). (B) Samples of whole saliva were irradiated with doses up to 5.1 kGy. Irradiation was performed in three independent experiments on samples from three individuals (data points are shown as •, ▴, and ▪). Each data point represents the result from one donor in one experiment. (C) Samples of whole saliva were filtrated in successive steps with membrane filters of decreasing pore size. Filtration was performed in two independent experiments on samples from two individuals (• and ▴). Each data point represents the result from one donor in one experiment. Aliquots of the samples were plated on agar, and bacterial colonies grown after 48 h were counted and calculated as CFU per ml of saliva. The detection limit was determined to be 20 CFU per ml of suspension and is indicated as a dashed horizontal line. Data below the limit of sensitivity were not considered for calculation of the D10 value or the SAL (insert in panel B).

Stefan Ruhl, et al. Appl Environ Microbiol. 2011 Feb;77(3):749-755.

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Support Center