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The upper-left panel shows the normalized ratio of nonsynonymous to synonymous variation for each functional segment of MUC7. The upper-right panel shows the overall nucleotide-level conservation for the same functional segments. The protein alignments of the signal peptide and the histatin-like domain that underlie these graphs are shown at the bottom. We did not include the PTS-repeat region in this analysis because copy number changes and sequence similarity introduce major alignment problems. P-values were calculated using a one-tailed Wilcoxon rank-sum test.