Colonization of the tooth surface by actinomyces and viridans group streptococci involves the attachment of these bacteria to adsorbed salivary components of the acquired enamel pellicle. The hypothesis that this attachment depends on specific adhesins has now been assessed from the binding of bacteria with well-defined adhesive properties to blots of SDS-PAGE-separated parotid and submandibular-sublingual (SM-SL) saliva. Streptococcus sanguis and type 2 fimbriated Actinomyces naeslundii, which bound terminal sialic acid and Galβ1-3GalNAc, respectively, recognized only a few SM-SL salivary components, primarily MG2. In contrast, type 1 fimbriated A. naeslundii and S. gordonii, which bound purified proline-rich proteins (PRPs), recognized several other components from both SM-SL and parotid saliva. Significantly, bacteria that lacked PRP-binding and the lectin-like activities detected by binding to MG2 failed to bind any immobilized salivary component. These findings suggest the involvement of specific adhesins in bacterial recognition of many adsorbed salivary proteins and glycoproteins.

Cisar JO (1986). Fimbrial lectins of the oral actinomyces. In: Microbial lectins and agglutinins: properties and biological activity. Mirelman D, editor. New York: John Wiley & Sons, Inc., pp. 183–196.
Cisar JO, David VA, Curl SH, Vatter AE (1984). Exclusive presence of lactose-sensitive fimbriae on a typical strain (WVU45) of Actinomyces naeslundii. Infect Immun 46:453–458. Medline
Cisar JO, Vatter AE, Clark WB, Curl SH, Hurst-Calderone S, Sandberg AL (1988). Mutants of Actinomyces viscosus T14V lacking type 1, type 2, or both types of fimbriae. Infect Immun 56:2984–2989. Medline
Cisar JO, Sandberg AL, Abeygunawardana C, Reddy GP, Bush CA (1995). Lectin recognition of host-like saccharide motifs in streptococcal cell wall polysaccharides. Glycobiology 5:655–662. Crossref, Medline
Cisar JO, Sandberg AL, Reddy GP, Abeygunawardana C, Bush CA (1997). Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins. Infect Immun 65:5035–5041. Medline
Clark WB, Beem JE, Nesbitt WE, Cisar JO, Tseng CC, Levine MJ (1989). Pellicle receptors for Actinomyces viscosus type 1 fimbriae in vitro. Infect Immun 57:3003–3008. Medline
Demuth DR, Golub EE, Malamud D (1990). Streptococcal-host interactions: structural and functional analysis of a Streptococcus sanguis receptor for a human salivary glycoprotein. J Biol Chem 265:7120–7126. Medline
Gibbons RJ (1989). Bacterial adhesion to oral tissues: a model for infectious diseases. J Dent Res 68:750–760. Link
Gibbons RJ, Etherden I, Moreno EC (1985). Contribution of stereochemical interactions in the adhesion of Streptococcus sanguis C5 to experimental pellicles. J Dent Res 64:96–101. Link
Gibbons RJ, Hay DI, Cisar JO, Clark WB (1988). Adsorbed salivary proline-rich protein 1 and statherin: receptors for type 1 fimbriae of Actinomyces viscosus T14V-J1 on apatitic surfaces. Infect Immun 56:2990–2993. Medline
Gibbons RJ, Hay DI, Schlesinger DH (1991). Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces. Infect Immun 59:2948–2954. Medline
Hsu SD, Cisar JO, Sandberg AL, Kilian M (1994). Adhesive properties of viridans streptococcal species. Microb Ecol Health Dis 7:125–137. Crossref
Kilian M, Nyvad B (1990). Ability to bind salivary α-amylase discriminates certain viridans streptococcal species. J Clin Microbiol 28:2576–2577. Medline
Lendenmann U, Grogan J, Oppenheim FG (2000). Saliva and dental pellicle—a review. Adv Dent Res 14:22–28. Link
Li T, Johansson I, Hay DI, Stromberg N (1999). Strains of Actinomyces naeslundii and Actinomyces viscosus exhibit structurally variant fimbrial subunit proteins and bind to different peptide motifs in salivary proteins. Infect Immun 67:2053–2059. Medline
Ligtenberg AJM, Walgreen-Weterings E, Veerman ECI, de Soet JJ, de Graaff J, Nieuw Amerongen AV (1992). Influence of saliva on aggregation and adherence of Streptococcus gordonii HG 222. Infect Immun 60:3878–3884. Medline
Murray PA, Levine MJ, Tabak LA, Reddy MS (1982). Specificity of salivary-bacterial interactions: II. Evidence for a lectin on Streptococcus sanguis with specificity for a NeuAcα2,3Galβ1,3GalNAc sequence. Biochem Biophys Res Commun 106:390–396. Crossref, Medline
Murray PA, Prakobphol A, Lee T, Hoover CI, Fisher SJ (1992). Adherence of oral streptococci to salivary glycoproteins. Infect Immun 60:31–38. Medline
Nyvad B, Kilian M (1987). Microbiology of the early colonization of human enamel and root surfaces in vivo. Scand J Dent Res 95:369–380. Medline
Oppenheim FG, Hay DI, Franzblau C (1971). Proline-rich proteins from human parotid saliva. I. Isolation and partial characterization. Biochemistry 10:4223–4238.
Palmer RJ Jr, Gordon SM, Cisar JO, Kolenbrander PE (2003). Coaggregation-mediated interactions of streptococci and actinomyces detected in initial human dental plaque. J Bacteriol 185:3400–3409. Crossref, Medline
Prakobphol A, Tangemann K, Rosen SD, Hoover CI, Leffler H, Fisher SJ (1999). Separate oligosaccharide determinants mediate interactions of the low-molecular-weight salivary mucin with neutrophils and bacteria. Biochemistry 38:6817–6825. Crossref, Medline
Prakobphol A, Xu F, Hoang VM, Larsson T, Bergström J, Johannsson I, et al. (2000). Salivary agglutinin, which binds Streptococcus mutans and Helicobacter pylori, is the lung scavenger receptor cysteine-rich protein gp-340. J Biol Chem 275:39860–39866. Crossref, Medline
Rogers JD, Palmer RJ Jr, Kolenbrander PE, Scannapieco FA (2001). Role of Streptococcus gordonii amylase-binding protein A in adhesion to hydroxyapatite, starch metabolism, and biofilm formation. Infect Immun 69:7046–7056. Crossref, Medline
Ruhl S, Sandberg AL, Cisar JO (1996). Recognition of immunoglobulin A1 by oral actinomyces and streptococcal lectins. Infect Immun 64:5421–5424. Medline
Ruhl S, Cisar JO, Sandberg AL (2000). Identification of polymorphonuclear leukocyte and HL-60 cell receptors for adhesins of Streptococcus gordonii and Actinomyces naeslundii. Infect Immun 68:6346–6354. Crossref, Medline
Sandberg AL, Mudrick LL, Cisar JO, Metcalf JA, Malech HL (1988). Stimulation of superoxide and lactoferrin release from polymorphonuclear leukocytes by the type 2 fimbrial lectin of Actinomyces viscosus T14V. Infect Immun 56:267–269. Medline
Scannapieco FA, Bergey EJ, Reddy MS, Levine MJ (1989). Characterization of salivary α-amylase binding to Streptococcus sanguis. Infect Immun 57:2853–2863. Medline
Scannapieco FA, Torres GI, Levine MJ (1995). Salivary amylase promotes adhesion of oral streptococci to hydroxyapatite. J Dent Res 74:1360–1366. Link
Takahashi Y, Sandberg AL, Ruhl S, Muller J, Cisar JO (1997). A specific cell surface antigen of Streptococcus gordonii is associated with bacterial hemagglutination and adhesion to α2-3-linked sialic acid-containing receptors. Infect Immun 65:5042–5051. Medline
Takahashi Y, Konishi K, Cisar JO, Yoshikawa M (2002a). Identification and characterization of hsa, the gene encoding the sialic acid-binding adhesin of Streptococcus gordonii DL1. Infect Immun 70:1209–1218. Crossref, Medline
Takahashi Y, Ruhl S, Yoon Y-W, Sandberg AL, Cisar JO (2002b). Adhesion of viridans group streptococci to sialic acid-, galactose- and N-acetylgalactosamine-containing receptors. Oral Microbiol Immunol 17:257–262. Crossref, Medline

Vol 83, Issue 6, 2004

Recommended Citation

Salivary Receptors for the Proline-rich Protein-binding and Lectin-like Adhesins of Oral Actinomyces and Streptococci

S. Ruhl, 1, A.L. Sandberg, 2, J.O. Cisar, *Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, Building 30, Room 532, National Institutes of Health, Bethesda, MD 20892;

Journal of Dental Research

Vol 83, Issue 6, pp. 505 - 510

First published date: November-20-2016

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