University at Buffalo - The State University of New York
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Histology Core - Medical Scientist Training Program - University at Buffalo
University at Buffalo - The State University of New York
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Core Facilities

Histology Core

John E. Tomaszewski, MD

John E. Tomaszewski, MD, who has expertise in histology, has translational research interests in genitourinary pathology.

Histology Core Facility Temporarily Closed

All services ceased Friday, September 29th, 2017.

We anticipate re-opening in January, 2018

Services

We provide histopathology services to students, faculty, researchers and clinicians at the University at Buffalo and in the larger Western New York community. Our facility can process tissue for paraffin embedding, section paraffin blocks and perform a wide variety of staining techniques.

We have two cryostats for sectioning frozen tissue specimens to facilitate rapid diagnosis, lipid and enzyme demonstration, certain immunohistochemical techniques and immunofluorescence work.

We collaborate with, train, advise and provide technical support for individuals in need of histology services for research, teaching or clinical interests. Please call us with questions about sample collection techniques to obtain optimal results from paraffin embedding and frozen section techniques.

Available Techniques

Paraffin Histology

This technique allows the preparation of sections as thin as three microns for routine and special histochemical staining, immunohistochemistry, in-situ hybridization, and PCR. Paraffin embedded tissues retain good morphology in archival blocks decades after the tissue was embedded.

Frozen Section Histology

Frozen section histology provides rapid results using unfixed tissue for techniques such as fat demonstration, immunohistochemistry, immunofluorescence and enzyme histochemistry. Most routine histologic stains can be performed on frozen tissue as well.

Immunohistochemistry (IHC)

We offer automated IHC with the Leica Bond, a 30-slide stainer. Our automated stainer delivers quality, efficiency and total tissue care with definition staining and minimal tissue loss. Investigators can supply their own antibodies to be developed with our current ancillary reagents. 

We are available to provide guidance on choosing appropriate antibodies and procedures for successful staining.

Serial Sections and Step Sections

Our technicians can prepare slides to display multiple sections from a tissue sample. Collecting every section creates a serial section; taking sections at specified depths from a block (e.g., every 50 microns) creates a step section.

Histologic Stains

  • H&E (hematoxylin and eosin): used for general morphology and reference.
  • Trichrome stains: show muscle tissue, fibrin, and collagen distinctly, often used to differentiate between fibrous and normal tissue.
  • PAS (periodic acid Schiff): a versatile stain, commonly used for polysaccharides, but it can be used for a variety of other tissue components such as mucin, hyaluronic acid, reticulin, fibrin of thrombi, colloid droplets, hyalin of arteriosclerosis, granular cells in renal arterioles, most basement membranes, colloid of pituitary stalks and thyroid tissue.
  • Silver stains: primarily used to identify molecules with strong reducing groups (e.g., melanin), to stain carbohydrates (e.g., glycoproteins), or localize cytoplasmic or extra-cytoplasmic structures that absorb silver ions (e.g., secretory granules of neuroendocrine cells).
  • Verhoff’s Elastic Stain: selectively stains the elastin fibers in tissue, useful to detect the loss of elasticity (from, e.g., emphysema or arterial thickening).

We can also perform special stains including Congo Red (for amyloid), Alcian Blue (acid mucopolysaccherides), Mucicarmine (mucin), Cresyl Violet (nerve cells and glia), Luxol Fast Blue (myelin), Prussian Blue (iron), and Giemsa and Wright’s (hematopoietic cells).

Location

218 Farber Hall

Hours

Monday to Friday
8 a.m. to 4 p.m.

Contact Information

Donna Carapetyan

Histology Technician

218 Farber Hall, Buffalo, NY 14214

Phone: (716) 829-3093

Email: UB-histolab@buffalo.edu