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P. gingivalis-induced alveolar bone loss in mice primed with ED1.
A & B, alveolar bone levels. A, Alveolar bone loss was assessed after 6 weeks by measuring the distance from the ABC to the CEJ at 14 maxillary buccal sites (7 sites on each side) per mouse and the total alveolar mean bone loss per group was plotted. B, Net bone loss induced by P. gingivalis in wild-type or ED1 primed groups was calculated as mean total ABC-CEJ distance of bacterially infected group minus mean total ABC-CEJ distance of the sham-infected group. C & D, osteoclastic activity measured as numbers of TRAP positive cells. C, Representative histological sections showing TRAP+ cells (indicated with yellow arrows) lining the bone crest in each group. (D) Average number of TRAP+ cells in 10 high power magnification fields/slide (4 mice/group). Data are from one of two independent experiments with similar results (mean ± s.d, of six mice in A & B or four mice in C & D; *, p<0.05).