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Effect of laser on TNF-alpha expression in inflamed human gingival tissue | SpringerLink

Lasers in Medical Science

, Volume 27, Issue 2, pp 377–381 | Cite as

Effect of laser on TNF-alpha expression in inflamed human gingival tissue

  • Snezana Pesevska
  • Marija Nakova
  • Icko Gjorgoski
  • Nikola Angelov
  • Kiro Ivanovski
  • Salvador Nares
  • Sebastiano Andreana
Original Article


This study sought to evaluate the effect of low-level laser treatment combined with scaling and root planing (SRP) on gingival tissue levels of TNF-alpha in subjects with periodontal disease. Eighty gingival papilla biopsy samples were obtained from 60 patients diagnosed with chronic advanced periodontitis; randomly assigned to three treatment groups (n = 20), as well as 20 subjects with no periodontal disease (group A). Group B received SRP on a single quadrant/day for four consecutive days. On day 5, all quadrants were rescaled. Groups C and D received the same treatment as group B plus laser application with the low-level diode laser (630–670 nm, 1.875 J/cm2) for five and ten consecutive days, respectively. Papilla biopsies were obtained from subjects and evaluated by ELISA for levels of TNF-alpha. The values in the control group were 5.2 ± 3.21 pg/mg and baseline values for the examined groups were 46.01 ± 16.69. Significantly decreased level of TNF-alpha for groups C and D was found after treatment, while group B demonstrated reduction of TNF-alpha of 31.34%. The results of this study show suppression of TNF-alpha in gingival tissue after low-level laser treatment as adjunct to SRP. Data may suggest beneficial anti-inflammatory effects of the laser treatment when used as adjunctive periodontal treatment.


Laser TNF-alpha Gingival tissue Periodontitis 


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Copyright information

© Springer-Verlag London Ltd 2011

Authors and Affiliations

  • Snezana Pesevska
    • 1
  • Marija Nakova
    • 1
  • Icko Gjorgoski
    • 2
  • Nikola Angelov
    • 3
  • Kiro Ivanovski
    • 1
  • Salvador Nares
    • 4
  • Sebastiano Andreana
    • 5
  1. 1.Faculty of DentistryUniversity Sts Cyril and MethodiusSkopjeMacedonia
  2. 2.Institute of Biology, Faculty of Natural Sciences and MathematicsUniversity Sts Cyril and MethodiusSkopjeMacedonia
  3. 3.Loma Linda University School of DentistryLindaUSA
  4. 4.University of North Carolina at Chapel HillChapel HillUSA
  5. 5.University at Buffalo, School of Dental MedicineBuffaloUSA

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