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PAK-PBD pulldown assays were used to quantify active Rac1 in neutrophils in response to fMLP. Msp-mediated Rac1 inhibition in response to fMLP was reversed by treatment of cells with VO-OHpic. A) A representative immunoblot of active Rac1 quantification is shown. B) Graph of densitometry analysis represents mean ± SEM (n = 3, * P<0.05, ** P<0.01, unpaired t test).