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1.
Fig. 4

Fig. 4. From: Absence of Capsule Reveals Glycan-Mediated Binding and Recognition of Salivary Mucin MUC7 by Streptococcus pneumoniae.

Streptococcal adhesion to immobilized WS and ductal glandular secretions (SMSL, PAR), naturally occurring glycoproteins and related neoglycoproteins. Suspensions of fluorochrome-labeled S. gordonii DL1, capsule-free S. pneumoniae TIGR4 mutant strain HR1001.1, and the respective isogenic SRR adhesin-deficient mutants were overlaid on dot blots of immobilized naturally occurring glycoproteins () and related neoglycoproteins (). Fluorescent signals of bound bacteria were detected by use of a fluorescence scanner.

Supaporn Thamadilok, et al. Mol Oral Microbiol. ;31(2):175-188.
2.
Fig. 1

Fig. 1. From: Absence of Capsule Reveals Glycan-Mediated Binding and Recognition of Salivary Mucin MUC7 by Streptococcus pneumoniae.

Streptococcal adhesion to immobilized whole saliva, parotid saliva and submandibular/sublingual saliva. Suspensions of fluorochrome-labeled S. gordonii DL1, S. sanguinis SK36, S. pneumoniae wild-type strain TIGR4, capsule-free mutant strain HR1001.1, and the respective isogenic SRR adhesin-deficient mutants were overlaid on nitrocellulose membranes carrying immobilized untreated (Ø) or sialidase-treated (S) dots (1 μg of total protein per dot) of whole saliva (WS), parotid saliva (PAR), and submandibular/sublingual saliva (SMSL). Fetuin and bovine serum albumin (BSA) were included as controls. Fluorescent signals of bound bacteria were detected by use of a fluorescence scanner.

Supaporn Thamadilok, et al. Mol Oral Microbiol. ;31(2):175-188.
3.
Fig. 2

Fig. 2. From: Absence of Capsule Reveals Glycan-Mediated Binding and Recognition of Salivary Mucin MUC7 by Streptococcus pneumoniae.

Streptococcal binding to salivary mucin MUC7. Untreated (Ø) or sialidase-treated (S) whole saliva (WS) as well as parotid (PAR), and submandibular/sublingual glandular secretions (SMSL) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and overlaid with fluorochrome-labeled bacteria. A, Periodic acid-Schiff stain to reveal the glycosylated mucin bands. B, Immunoblot with anti-MUC7 antibody. C, Bacterial overlay with fluorochrome-labeled S. gordonii DL1, S. sanguinis SK36, encapsulated S. pneumoniae wild-type strain TIGR4, capsule-free mutant strain HR1001.1, and respective isogenic SRR adhesin-deficient mutants. Fluorescent signals of bound antibodies or bound bacteria were detected using a fluorescence scanner.

Supaporn Thamadilok, et al. Mol Oral Microbiol. ;31(2):175-188.
4.
Fig. 3

Fig. 3. From: Absence of Capsule Reveals Glycan-Mediated Binding and Recognition of Salivary Mucin MUC7 by Streptococcus pneumoniae.

Streptococcal-mediated hemagglutination of human red blood cells. Two-fold serial dilutions of S. gordonii DL1, S. sanguinis SK36, S. pneumoniae wild-type strain TIGR4, capsule-free mutant strain HR1001.1, and respective isogenic SRR adhesin-deficient mutants were mixed and incubated with human red blood cells. The sialic acid specific lectin from Maackia amurensis (MAL II) was used as a control for the presence or loss of sialic acid residues. A, Hemagglutination done with untreated (Ø) or sialidase-treated (S) RBCs. B, Treatment of RBCs with 2 mM or 10 mM sodium periodate to oxidize sialic acids or general glycans, respectively, prior to hemagglutination.

Supaporn Thamadilok, et al. Mol Oral Microbiol. ;31(2):175-188.

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