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20230631[PMID] - PMC - NCBI

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1.
Figure 3

Figure 3. From: Characterization of extracellular matrix macromolecules in primary cultures of equine keratinocytes.

Expression of hemidesmosome components in equine keratinocytes. Immunofluorescence images of skin (A) and lamellar (B) keratinocytes and dermal fibroblasts (C) labelled for integrin α6 and proteins of the hemidesmosome complex. Cells were grown on coverslips, fixed and labelled with each antibody. Inset in skin keratinocytes panel A demonstrates integrin α6 localization in sub-confluent cells compared to confluent cells in larger image. Images are representative of three independent experiments. Scale bar = 20 μm.

Michelle B Visser, et al. BMC Vet Res. 2010;6:16-16.
2.
Figure 1

Figure 1. From: Characterization of extracellular matrix macromolecules in primary cultures of equine keratinocytes.

Characterization of equine keratinocytes. A. Immunofluorescence analysis of cytokeratin and vimentin expression in equine keratinocytes. Lamellar cells were grown in both high and low calcium conditions while skin keratinocytes are grown only in high calcium conditions. Nuclear staining with DAPI is shown for skin keratinocytes in middle panel, as these cells stained negative for vimentin Scale bar = 30 μm. Cell morphology images are phase contrast photographs of skin and lamellar cells. Scale bar = 10 μm. Images are representative of 3 independent experiments. B. Immunoblots of skin and lamellar whole cell lysates demonstrating expression of cytokeratins detected using antibody AE1/AE3 which recognizes both acidic and basic groups of cytokeratins. HeLa cell and equine skin fibroblast lysates are used as positive and negative controls, respectively, for cytokeratin expression. Images are representative of two independent experiments.

Michelle B Visser, et al. BMC Vet Res. 2010;6:16-16.
3.
Figure 2

Figure 2. From: Characterization of extracellular matrix macromolecules in primary cultures of equine keratinocytes.

Ln-332 synthesis and processing in equine keratinocytes. A. Immunofluorescence analysis of expression of Ln-332 α3β3γ2 and individual Ln-332 subunits in skin and lamellar keratinocytes. Scale bar = 10 μm. Inset in skin keratinocytes panel A demonstrates Ln-332 α3β3γ2 localization in sub confluent cells compared to confluent cells in larger image. Images of equine fibroblasts stained with each antibody serves as a negative control. As these cells do not produce Ln-332, nuclear staining with DAPI is shown in each panel. B. Immunoblots of individual Ln-332 subunit forms present in cell and matrix layers as well as secreted media from equine keratinocytes. Aliquots of acetone precipitated conditioned media, cell layers and matrix preparations were analyzed with each Ln-332 subunit antibody. Serum free conditioned media of the human squamous cell line SCC25 and the mouse epidermal cell lime Pam212 serve as positive controls for Ln-332 subunit molecular weight and processing. C. Confocal microscopy of equine keratinocytes grown on coverslips labelled for Ln-332 α3β3γ2 localization. Images are shown from both the basal and middle section of the cell monolayer. Scale bar = 10 μm.

Michelle B Visser, et al. BMC Vet Res. 2010;6:16-16.

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