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Bacterial interactions with separated erythrocytes, platelets, and whole human plasma.
(A) Wild type bacteria only hemagglutinate non-sialidase treated RBCs. Mutant strains do not show any hemagglutination. Bars represent median values. (n = 3). (B) Bacterial adhesion to fixed, immobilized platelets. (n = 6, SEM). (C) Whole plasma samples were immobilized on dot blot in three-fold serial dilutions starting from the original concentration in whole blood. Each strain was tested to interact with either non-sialidase treated (Ø) or sialidase treated whole plasma (S). *P<0.05, ***P<0.001.