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A model for the action of Msp in subcortical actin filament assembly in fibroblasts.
Msp treatment of fibroblasts results in activation of both PI3K and a host 3-phosphoinositide phosphatase, resulting in turnover of PIP3 to PIP2. PIP2 then causes removal of actin capping proteins, FBE formation and dependent actin filament assembly, even though cofilin remains inactive. Although Msp activates Rac1, RhoA and Ras, our data suggest that these small GTPases are not directly involved in Msp-induced subcortical actin assembly. indicates pathway activated, indicates pathway inhibited.