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The Clinical Proteomic Technologies for Cancer | Details for Glutathione S-Transferase Mu1

Glutathione S-Transferase Mu1


Background

Catalog Number:

CPTC-GSTMu1-1

Target Antigen:

Glutathione S-Transferase Mu1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

03/07/2013

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-GSTMu1-1 as primary Ab against Ag 10541 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu1-1 Western Blot

Western Blot using CPTC-GSTMu1-1 as primary Ab against Ag 10541 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity measured by indirect ELISA with the GST group of antigens.  Click image to enlarge

Cross Reactivity Data

This table shows the cross reactivity measured by indirect ELISA with the GST group of antigens.

Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-GSTMu1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-GSTMu1-1 Protein Array

Protein Array in which CPTC-GSTMu1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

  IHC NCI60
Click to enlarge image Immunohistochemistry of CPTC-GSTMu1-1 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:4000 Click image to enlarge

CPTC-GSTMu1-1 IHC NCI60

Immunohistochemistry of CPTC-GSTMu1-1 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:4000

Click to view IHC NCI60 Cell Line Array example
Close

IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-GSTMu1-1. Titer: 1:4000 Click image to enlarge

CPTC-GSTMu1-1 IHC Tissue

Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-GSTMu1-1. Titer: 1:4000

  IHC HPA
Download CPTC-GSTMu1-1.pdf (93.5 KB)

CPTC-GSTMu1-1 Evaluation by Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Background

Catalog Number:

CPTC-GSTMu1-2

Target Antigen:

Glutathione S-Transferase Mu1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

03/07/2013

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-GSTMu1-2 as primary Ab against Ag 10541 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu1-2 Western Blot

Western Blot using CPTC-GSTMu1-2 as primary Ab against Ag 10541 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu1-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens.  Click image to enlarge

Cross Reactivity Data

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.

Characterization SOP Files

  NCI 60 Protein Array

CPTC-GSTMu1-2 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC NCI60

CPTC-GSTMu1-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Close

IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue

CPTC-GSTMu1-2 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC HPA
Download CPTC-GSTMu1-2.pdf (101.1 KB)

CPTC-GSTMu1-2

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Background

Catalog Number:

CPTC-GSTMu1-3

Target Antigen:

Glutathione S-Transferase Mu1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

03/07/2013

Antigen Recognition(s):

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-GSTMu1-3 as primary Ab against Ag 10541 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu1-3 Western Blot

Western Blot using CPTC-GSTMu1-3 as primary Ab against Ag 10541 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu1-3 ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens.  Click image to enlarge

Cross Reactivity Data

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.

Characterization SOP Files

  NCI 60 Protein Array

CPTC-GSTMu1-3 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC NCI60

CPTC-GSTMu1-3 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Close

IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue

CPTC-GSTMu1-3 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC HPA
Download CPTC-GSTMu1-3.pdf (105.5 KB)

CPTC-GSTMu1-3 Evaluation by Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Background

Catalog Number:

CPTC-GSTMu1-4

Target Antigen:

Glutathione S-Transferase Mu1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

03/07/2013

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-GSTMu1-4 as primary Ab against Ag 10541 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu1-4 Western Blot

Western Blot using CPTC-GSTMu1-4 as primary Ab against Ag 10541 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu1-4 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens.  Click image to enlarge

Cross Reactivity Data

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.

Characterization SOP Files

  NCI 60 Protein Array

CPTC-GSTMu1-4 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC NCI60

CPTC-GSTMu1-4 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Close

IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue

CPTC-GSTMu1-4 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC HPA
Download CPTC-GSTMu1-4.pdf (95.5 KB)

CTPC-GSTMu1-4 Evaluation by Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Background

Catalog Number:

CPTC-GSTMu1-5

Target Antigen:

Glutathione S-Transferase Mu1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

12/15/2011

Antigen Recognition(s):

recombinant full-length

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-GSTMu1-5 as primary Ab against Ag 10460 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu1-5 Western Blot

Western Blot using CPTC-GSTMu1-5 as primary Ab against Ag 10460 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu1-5 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens.  Click image to enlarge

Cross Reactivity Data

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.

Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-GSTMu1-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-GSTMu1-5 Protein Array

Protein Array in which CPTC-GSTMu1-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

  IHC NCI60
Click to enlarge image Immunohistochemistry of CPTC-GSTMu1-5 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:3000 Click image to enlarge

CPTC-GSTMu1-5 IHC NCI60

Immunohistochemistry of CPTC-GSTMu1-5 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:3000

Click to view IHC NCI60 Cell Line Array example
Close

IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-GSTMu1-5. Titer: 1:3000 Click image to enlarge

CPTC-GSTMu1-5 IHC Tissue

Tissue Micro-Array(TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-GSTMu1-5. Titer: 1:3000

  IHC HPA
Download GSTM1-5 HPA Report.pdf (138.2 KB)

CPTC-GSTMu1-5 evaluation by the Human Protein Atlas

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).

Background

Catalog Number:

CPTC-GSTMu1-6

Target Antigen:

Glutathione S-Transferase Mu1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

03/07/2013

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-GSTMu1-6 as primary Ab against Ag 10460 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu1-6 Western Blot

Western Blot using CPTC-GSTMu1-6 as primary Ab against Ag 10460 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu1-6 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens.  Click image to enlarge

Cross Reactivity Data

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.

Characterization SOP Files

  NCI 60 Protein Array

CPTC-GSTMu1-6 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC NCI60

CPTC-GSTMu1-6 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Close

IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue

CPTC-GSTMu1-6 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC HPA
Download CPTC-GSTMu1-6.pdf (98.0 KB)

CPTC-GSTMu1-6 Evaluation by Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Background

Catalog Number:

CPTC-GSTMu1-7

Target Antigen:

Glutathione S-Transferase Mu1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

03/07/2013

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-GSTMu1-7 as primary Ab against Ag 10460 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu1-7 Western Blot

Western Blot using CPTC-GSTMu1-7 as primary Ab against Ag 10460 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu1-7 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens.  Click image to enlarge

Cross Reactivity Data

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.

Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-GSTMu1-7 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-GSTMu1-7 Protein Array

Protein Array in which CPTC-GSTMu1-7 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

  IHC HPA
Download CPTC-GSTMu1-7.pdf (102.8 KB)

CPTC-GSTMu1-7 Evaluation by Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Background

NCI Identification Number:

10541

Antigen Name:

Glutathione S-Transferase Mu1

CPTC Name:

CPTC-GSTMu1

Keywords:

GSTM1; EC 2.5.1.18; GST1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; MGC26563; MU; MU-1; OTTHUMP00000013346; OTTHUMP00000013347

Function:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles

Chromosomal Localization:

1p13.3

Expression System:

E. Coli

NCBI Accession Number:

J03817

Swiss Prot Accession Number:

P09488

DNA Source:

HIP:HsCD00005373

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

40005

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMPMILGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYD
RSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLC
GETEEEKIRVDILENQTMDNHMQLGMICYNPEFEKLKPKYLEELP
EKLKLYSEFLGKRPWFAGNKITFVDFLVYDVLDLHRIFEPNCLDA
FPNLKDFISRFEGLEKISAYMKSSRFLPRPVFSKMAVWGNK

Native Sequence:

MPMILGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRS
QWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCG
ETEEEKIRVDILENQTMDNHMQLGMICYNPEFEKLKPKYLEELP
EKLKLYSEFLGKRPWFAGNKITFVDFLVYDVLDLHRIFEPNCLD
AFPNLKDFISRFEGLEKISAYMKSSRFLPRPVFSKMAVWGNK

Calculated Isoelectric Point:

6

Molecular Weight:

25970

Last Updated:

11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Protein Array in which CPTC-GSTMu1-7 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click image to enlarge

CPTC-GSTMu1

PAGE of Ag 10541 with molecular weight standards in lane 1.

SOPs

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