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PubMed Central, Figure 6: Sci Rep. 2017; 7: 5747. Published online 2017 Jul 18. doi:  10.1038/s41598-017-06126-x
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Published online 2017 Jul 18. doi:  10.1038/s41598-017-06126-x

Figure 6

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Bone marrow effector CD4+ T-cell hematopoiesis. (ac) Marrow Transcription Factor Expression Assays: 12 week-old male SPF & GF mice were euthanized; femoral bone marrow cells were isolated and stained (n = 4/gp) for flow cytometric analysis to assess (a) % CD4+FOXP3+ (TREG) cells, (b) % CD4+RORγt+ (TH17) cells, and (c) % CD4+T-bet+ (TH1) cells. Percentages are expressed relative to CD4+ cells. (dg) Marrow Intracellular Cytokine Expression Assays: 11 week-old gender matched (2 male and 2 female per group) SPF & GF mice were euthanized; femoral whole marrow was plated overnight for cytokine activation (PMA, Ionomycin, Monensin). Cells were isolated and stained (n = 4/gp) for flow cytometric analysis to assess (d) % CD3+CD4+CD8IL10+IL17a (CD4+IL10+) cells, (e) % CD3+CD4+CD8IL10IL17a+ (CD4+IL17a+) cells, (f) % CD3+CD4+CD8IFNγIL17a+ (CD4+IL17a+) cells, and (g) % CD3+CD4+CD8IFNγ+IL17a (CD4+IFNγ+) cells. Percentages are expressed relative to CD3+CD4+CD8 cells. Data are reported as mean ± SEM. *p < 0.05 vs. SPF; **p < 0.01 vs. SPF.

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