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PubMed Central, Figure 2: Mucosal Immunol. 2013 Sep; 6(5): 972–984. Published online 2013 Jan 9. doi:  10.1038/mi.2012.135
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PMC full text:
Mucosal Immunol. Author manuscript; available in PMC 2014 Mar 1.
Published in final edited form as:
Mucosal Immunol. 2013 Sep; 6(5): 972–984.
Published online 2013 Jan 9. doi:  10.1038/mi.2012.135

Figure 2

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IL-17, but not IFNγ is crucial for vaccine-induced mucosal immunity against Mtb infection

B6, Ifng−/− and Il17−/− were left unvaccinated (Un) or mucosally vaccinated (Vacc) with ESAT61–20 in combination with LT-IIb and rested for 30 days. Subsequently mice were challenged with ~100 CFU Mtb by the aerosol route and lung bacterial burden was determined on day 30 post infection (a), day 45 (b) or day 60 (c). The number of cytokine-producing ESAT61–20-specific CD4+ T cells in the lungs were determined by ELISpot in B6 and Ifng−/− (d), B6 and Il17−/− (e) mucosally vaccinated Mtb-challenged mice on day 15 post infection. B6 mice were mucosally vaccinated, rested for 30 days and challenged with Mtb, following which they were treated with isotype control antibody or IL-17 neutralizing antibody between day 5 and 21 (100 µg/mouse every 48 hours), and lung bacterial burden was determined on day 30 post-infection (f). Stat3.Cd4−/− mice or control littermates (Control) were vaccinated with ESAT61–20 in HLTs or left unvaccinated and number of cytokine-producing ESAT61–20-specific CD4+ T cells in the lungs were determined by ELISpot on day 15 post mucosal vaccination (g). Mucosally vaccinated Stat3.Cd4−/− mice or control littermate (Control) mice were rested for 30 days and challenged with ~100 CFU Mtb by the aerosol route and lung bacterial burden was determined on day 30 post infection (h). Ifng−/− mice were vaccinated, rested for 30 days, challenged with Mtb following which they were treated with isotype control antibody or IL-17 neutralizing antibody between day 5 and 21 (100 µg/mouse every 48 hours), and the lung bacterial burden was determined on day 30 post infection (i). The data points represent the mean (±SD) of values from 4–6 mice (a–i). *, p ≤0.05. **, p ≤0.005. ***, p ≤0.0005, ns-not significant. One of two independent experiments shown.

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