DING XU, PhD personal profile
The main interest of the lab is to understand how proteins
interact with heparan sulfate (HS), a highly negatively charged
linear polysaccharide, and the physiological significance of the
interactions in bone remodeling and inflammation. Universally
expressed by all mammalian cells, HS is a major and often dominant
component of the landscape at the cell surface and thus plays
essential roles in cell signaling, cell-cell interactions and
host-pathogen interactions. At the cell surface, HS interacts with
hundreds of HS-binding proteins, many of which are important drug
targets (such as TNF-alpha, interleukin-8 and FGF receptors). The
dependence of these proteins on HS for normal function suggests
that disrupting HS-protein interaction could be an effective means
to block the activity of these HS-binding proteins.
The goal of the lab is to dissect the structural determinants of HS-protein interactions by characterizing structures of HS/protein complexes (using X-ray crystallography and small-angle scattering), and to use this information to develop and test the pre-clinical efficacy of novel therapeutic agents (including monoclonal antibodies, oligosaccharides and small molecules) that target HS-protein interactions in cellular and animal models. The HS-binding proteins we are currently working on include receptor for advanced glycation end products (RAGE) and osteoprotegerin.