<<<<< << <<< /> or progression, suggesting a role for this protein as or progression, suggesting a role for this protein asor progression, suggesting a role for this protein as or progression, suggesting a role for this protein as a bor progression, suggesting a role for this protein as a biomor progression, suggesting a role for this protein asor progression, suggesting a role for this protein as a bor progression, suggesting a role for this protein as a bioor progression, suggesting a role for this protein as a or progression, suggesting a role for this protein as a biomor progression, suggesting a role for this protein as a bioor progression, suggesting a role for this protein as a bior progression, suggesting a role for this protein as a biomarker for early N-stage, aggressive SCCs. [Cancer Res 2008;68(10):3959?69]" /> or Rap1GAP, cell invasion and MMP secreti or Rap1GAP, cell invasion and MMP secretion were determined or Rap1GAP, cell invasion and MMP secretion were determined or Rap1GAP, cell invasion and MMP secretion were determined by Matrigel assays and gelatin zymography, respectively. Rap1GAP up-regulated transcription and secretion of MMP2 and MMP9, as assayed by q or Rap1GAP, cell invasion and MMP secretion were determined by Matrigel assays and gelatin zymography, respectively. Rap1GAP up-regulated transcription and secretion of MMP2 and MMP9, as assayed by quantitative reverse transcription-PCR and zymography. Furthermore, chemical and RNA interference blockade of MMP2/MMP9 inhibited invasion by Rap1GAP-transfected cells. Immunohistochemical staining of a human oropharyngeal SCC tissue microarray showed that Rap1GAP and MMP9 expression and staining intensity are correlated ( P < 0.0001)ell cycle. However, cells transfected with Rap1GAP exhibit a more invasive phenotype than corresponding vector-transfected control cells. MMP2 and MMP9 are enzymes that mediate SCC invasion via degradation of the extracellular matrix. Using SCC cells transfected with empty vector or Rap1GAP, cell invasion and MMP secretion were determined by Matrigel assays and gelatin zymography, respectively. Rap1GAP up-regulated transcription and secretion of MMP2 and MMP9, as assayed by quantitative reverse transcription-PCR and zymography. Furthermore, chemical and RNA interference blockade of MMP2/MMP9 inhibited invasion by Rap1GAP-transfected cells. Immunohistochemical staining of a human oropharyngeal SCC tissue microarray showed that Rap1GAP and MMP9 expression and staining intensity are correlated (<i>P</i> &lt; 0.0001) and that, in early N-stage lesions of SCC, high MMP9 is prognostic of poor disease-specific survival (<i>P</i> &lt; 0.05). Furthermore, Rap1GAP staining is correlated with MMP2 (<i>P</i> &lt; 0.03). MMP2 in combination with N stage has a prognostic effect on time to indication of surgery at primary site. MMP2 intensity is also positively correlated with T stage (<i>P</i> &lt; 0.015). In conclusion, Rap1GAP inhibits tumor growth but induces MMP2- and MMP9-mediated SCC invasion and tumor progression, suggesting a role for this protein as a biomarker for early N-stage, aggressive SCCs. [Cancer Res 2008;68(10):3959?69]</p>" /> ell cycle. However, cells transfected with Rap1GAP exhibit a moreell cycle. However, cells transfected with Rap1GAP exhibit a more invasive phenotype tell cycle. However, cells transfected with Rap1GAP exhibit a moell cycle. However, cells transfected with Rap1GAP exhibitell cycle. However, cells transfected with Rap1GAell cycle. However, cells transfected with Rap1GAP exhibit a more invasive phenotype than corresponell cycle. However, cells transfected with Rap1GAP exhibit a more invasive phenotype than correspondinabstract" /> abstract" /> c0750" /> itation_issn=0031-9333;citation_pmid=11152757" /> -0625.2003.120201.x" /> ch;citation_journal_abbrev=Clin. Cancer Res.;citation_title=Overexpression of Matrix Metalloproteinase-1 and -9 mRNA Is Associated with Progression of Oral Dysplasia to Cancer;citation_pages=6460-6465;citation_volume=10;citation_year=2004;citation_issue=19;citation_issn=1078-0432;citation_pmid=15475433;citation_doi=10.1158/1078-0432.CCR-04-0656" /> ;citation_title=An orthotopic floor-of-mouth model for locoregional growth and spread of human squamous cell carcinoma.;citation_pages=363-370;citation_volume=36;citation_year=2007;citation_issue=6;citation_pmid=17559499;citation_doi=10.1111/j.1600-0714.2007.00549.x" /> es sur le cancer;citation_journal_abbrev=Recent Results Cancer Res;citation_title=Induction chemotherapy for organ preservation in advanced squamous cell carcinoma of the oral cavity and oropharynx.;citation_pages=133-143;citation_volume=134;citation_year=1994;citation_pmid=8153429" /> loproteinases in squamous cell carcinomas of the head and neck.;citation_pagPathology;citation_journal_abbrev=Am. J. Pathol.;citation_title=Co-Expression of p16INK4A and Laminin 5 {gamma}2 by Microinvasive and Superficial Squamous Cell Carcinomas in Vivo and by Migrating Wound and Senescent Keratinocytes in Culture;citation_pages=477-491;citation_volume=163;citation_year=2003;citation_issue=2;citation_issn=0002-9440;citation_pmid=12875969" /> Pathology;citation_journal_abbrev=Am. J. Pathol.;citation_title=Co-Expression of p16INK4A and Laminin 5 {gamma}2 by Microinvasive and Superficial Squamous Cell Carcinomas in Vivo and by Migrating Wound and Senescent Keratinocytes in Culture;citation_pages=477-491;citation_volume=163;citation_year=2003;citation_issue=2;citation_issn=0002-9440;citation_pmid=12875969" /> n_volume=88;citation_year=2000;citation_issue=10;citation_pmid=10820340;citation_doi=10.1002/(SICI)1097-0142(20000515)88:10<2201::AID-CNCR2>3.0.CO;2-N" /> 2-128;citation_volume=85;citation_year=2001;citation_issue=1;citation_pmid=11437413;citation_doi=10.1054/bjoc.2001.1862" /> es;citation_title=Matrix metalloproteinases expressed in squamous cell carcinoma of the oral cavity: correlation with clinicopathologic features and neo-adjuvant chemothes;citation_title=Matrix metalloproteinases expressed in squamous cell carcinoma of the oral cavity: correlation with clinicopathologic features aes;citation_title=Matrix metalloproteinases expressed in squamous cell carcinoma of the oral cavity: correlation with clinicopathologic features and neo-adjuvant chemotherapy response.;citation_pages=279-284;citation_volume=18;citation_year=1999;citation_issue=3;citation_pmid=10606169" /> eee/////cdn.jsdelivr.net/qtip2/2.2.1/jquery.qtip.min.css" media="all" /> See cccccc pe)",pe)","wide":"all and (min-width: 1220px)"}}}}); //--> pe)","wide":"all and (min-width: 1220px)"}}}}); //--> r-menu-dropdown">