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PubMed Central, Figure 6: Cell Death Differ. 2016 June; 23(6): 1073–1085. Published online 2016 January 15. doi: 10.1038/cdd.2015.162
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Published online 2016 January 15. doi: 10.1038/cdd.2015.162

Figure 6

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ΔNp63 expression in lesional skin from atopic dermatitis subjects. (a) Upper panel shows hematoxylin and eosin (H&E) staining of normal human skin, AD non-lesional and lesional skin. Lower panel shows representative immunofluorescence staining of ΔNp63 isoform-specific expression in the epidermis of normal skin, AD non-lesional, AD lesional skin. Dotted white lines indicate the epidermal–dermal junction. Scale bar: 75 μm. (b) Quantification of the percentage of ΔNp63+ cells in normal human skin, AD non-lesional and lesional skin is shown. A total of 15 fields of view were used for quantification analysis on normal human skin (n=8), AD non-lesional skin (n=8) and AD lesional (n=8) skin samples. Data are represented as mean±S.D. (c) Quantitative RT-PCR analysis of mRNA expression levels of ΔNp63 and TAp63 in normal skin (n=5) and AD lesional skin (n=5). Values were normalized to the housekeeping gene GAPDH. Data are represented as mean±S.E. *P=0.001, Student's t-test

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