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17122351[PMID] - PMC - NCBI

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Items: 5

1.
FIG. 5.

FIG. 5. From: Identification of Genes Regulated by the cepIR Quorum-Sensing System in Burkholderia cenocepacia by High-Throughput Screening of a Random Promoter Library .

Comparison of the levels of expression of pBSCO1 (BCAL1040::luxCDABE) and pVDO301 (BCAL1696::luxCDABE) in K56-2 and K56cciIR. (A) pBSC01. Cultures were grown in LB medium containing 100 μg/ml trimethoprim. (B) pVDO301. Cultures were grown in TSB-DC medium containing 100 μg/ml trimethoprim. The values are means ± standard deviations for triplicate cultures.

Benchamas Subsin, et al. J Bacteriol. 2007 Feb;189(3):968-979.
2.
FIG. 4.

FIG. 4. From: Identification of Genes Regulated by the cepIR Quorum-Sensing System in Burkholderia cenocepacia by High-Throughput Screening of a Random Promoter Library .

Quantitative RT-PCR of genes expressed from the random promoter library. The mean relative expression levels of the target gene were normalized to the levels of ndh and multiplied by 1,000. Solid bars, K56-2; open bars, K56-dI2; gray bars, K56-R2. The values are means ± standard deviations for six replicates.

Benchamas Subsin, et al. J Bacteriol. 2007 Feb;189(3):968-979.
3.
FIG. 3.

FIG. 3. From: Identification of Genes Regulated by the cepIR Quorum-Sensing System in Burkholderia cenocepacia by High-Throughput Screening of a Random Promoter Library .

Expression of the ndh gene in B. cenocepacia K56-2, K56-dI2, and K56-R2. Total RNA was extracted from 8, 12, and 16 cultures and subjected to real-time RT-PCR. The absolute expression levels were determined using an ndh standard curve. The values are means ± standard deviations for triplicate assays. Solid bars, K56-2; open bars, K56-dI2; gray bars, K56-R2.

Benchamas Subsin, et al. J Bacteriol. 2007 Feb;189(3):968-979.
4.
FIG. 2.

FIG. 2. From: Identification of Genes Regulated by the cepIR Quorum-Sensing System in Burkholderia cenocepacia by High-Throughput Screening of a Random Promoter Library .

Comparison of the levels of expression of pvdA (pVDA01) and orbI::luxCDBAE (pVDO301) fusions in K56-2, K56-dI2, and K56-R2. Cultures were grown in TSB-DC medium containing 100 μg/ml trimethoprim. Dark gray bars, pVDA01; light gray bars, pVDO301. The values are means ± standard deviations for data obtained at 24 h for triplicate cultures and are representative of three experiments. Similar results were obtained at other times in the stationary phase.

Benchamas Subsin, et al. J Bacteriol. 2007 Feb;189(3):968-979.
5.
FIG. 1.

FIG. 1. From: Identification of Genes Regulated by the cepIR Quorum-Sensing System in Burkholderia cenocepacia by High-Throughput Screening of a Random Promoter Library .

Effects of various concentrations of OHL on expression of cepI::luxCDABE, zmpA::luxCDABE, and cepR::luxCDBAE fusions. Expression of promoter-luxCDABE fusions was measured throughout growth in LB medium containing 100 μg/ml trimethoprim supplemented with different concentrations of OHL. The values are the means for triplicate cultures. (A) cepI::luxCDABE. (B) zmpA::luxCDABE. (C) cepR::luxCDABE. ▪, K56-2; ▴, K56-dI2; •, K56-dI2 with 300 pM OHL; □, K56-dI2 with 30 pM OHL; ▵, K56-dI2 with 3 pM OHL; ▿, K56-dI2 with 300 fM OHL; ⋄, K56-dI2 with 30 fM OHL.

Benchamas Subsin, et al. J Bacteriol. 2007 Feb;189(3):968-979.

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