Curcumin is a plant-derived dietary spice ascribed various biological activities. Curcumin therapeutic applications have been studied in a variety of conditions, but not on periodontal disease. Periodontal disease is a chronic inflammatory condition initiated by an immune response to micro-organisms of the dental biofilm. Experimental periodontal disease was induced in rats by injecting LPS in the gingival tissues on the palatal aspect of upper first molars (30 µg LPS, 3 times/week for 2 weeks). Curcumin was administered to rats daily via oral gavage at 30 and 100 mg/kg body weight. Reverse transcriptase-qPCR and ELISA were used to determine the expression of IL-6, TNF-α and prostaglandin E2 synthase on the gingival tissues. The inflammatory status was evaluated by stereometric and descriptive analysis on hematoxylin/eosin-stained sections, whereas modulation of p38 MAPK and NK-κB signaling was assessed by Western blot. Curcumin effectively inhibited cytokine gene expression at mRNA and protein levels, but NF-κB was inhibited only with the lower dose of curcumin, whereas p38 MAPK activation was not affected. Curcumin produced a significant reduction on the inflammatory infiltrate and increased collagen content and fibroblastic cell numbers. Curcumin potently inhibits innate immune responses associated with periodontal disease, suggesting a therapeutic potential in this chronic inflammatory condition.

1. de Aquino SG, Guimaraes MR, Stach-Machado DR, da Silva JA, Spolidorio LC, Rossa CJr. Differential regulation of MMP-13 expression in two models of experimentally induced periodontal disease in rats. Arch Oral Biol 2009; 54: 609617. Crossref, Medline
2. Rogers JE, Li F, Coatney DD, . Actinobacillus actinomycetemcomitans lipopolysaccharide-mediated experimental bone loss model for aggressive periodontitis. J Periodontol 2007; 78: 550558. Crossref, Medline
3. Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol 2006; 71: 13971421. Crossref, Medline
4. Jagetia GC, Aggarwal BB. ‘Spicing up’ of the immune system by curcumin. J Clin Immunol 2007; 27: 1935. Crossref, Medline
5. Kim GY, Kim KH, Lee SH, . Curcumin inhibits immunostimulatory function of dendritic cells: MAPKs and translocation of NF-kappa B as potential targets. J Immunol 2005; 174: 81168124. Crossref, Medline
6. Gradisar H, Keber MM, Pristovsek P, Jerala R. MD-2 as the target of curcumin in the inhibition of response to LPS. J Leukoc Biol 2007; 82: 968974. Crossref, Medline
7. Lubbad A, Oriowo MA, Khan I. Curcumin attenuates inflammation through inhibition of TLR-4 receptor in experimental colitis. Mol Cell Biochem 2009; 322: 127135. Crossref, Medline
8. Chearwae W, Bright JJ. 15-deoxy-Delta(12,14)-prostaglandin J(2) and curcumin modulate the expression of Toll-like receptors 4 and 9 in autoimmune T lymphocyte. J Clin Immunol 2008; 28: 558570. Crossref, Medline
9. Gulcubuk A, Altunatmaz K, Sonmez K, . Effects of curcumin on tumour necrosis factor-alpha and interleukin-6 in the late phase of experimental acute pancreatitis. J Vet Med A Physiol Pathol Clin Med 2006; 53: 4954. Crossref, Medline
10. Gukovsky I, Reyes CN, Vaquero EC, Gukovskaya AS, Pandol SJ. Curcumin ameliorates ethanol and nonethanol experimental pancreatitis. Am J Physiol 2003; 284: G85G95.
11. Fu Y, Zheng S, Lin J, Ryerse J, Chen A. Curcumin protects the rat liver from CCl4-caused injury and fibrogenesis by attenuating oxidative stress and suppressing inflammation. Mol Pharmacol 2008; 73: 399409. Crossref, Medline
12. Surh YJ. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer 2003; 3: 768780. Crossref, Medline
13. Huang WT, Niu KC, Chang CK, Lin MT, Chang CP. Curcumin inhibits the increase of glutamate, hydroxyl radicals and PGE2 in the hypothalamus and reduces fever during LPS-induced systemic inflammation in rabbits. Eur J Pharmacol 2008; 593: 105111. Crossref, Medline
14. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1997; 388: 548554. Crossref, Medline
15. Mercurio F, Zhu H, Murray BW, . IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science 1997; 278: 860866. Crossref, Medline
16. Milward MR, Chapple IL, Wright HJ, Millard JL, Matthews JB, Cooper PR. Differential activation of NF-kappaB and gene expression in oral epithelial cells by periodontal pathogens. Clin Exp Immunol 2007; 148: 307324. Crossref, Medline
17. Carayol N, Chen J, Yang F, . A dominant function of IKK/NF-kappaB signaling in global lipopolysaccharide-induced gene expression. J Biol Chem 2006; 281: 3114231151. Crossref, Medline
18. Nakajima Y, Furuichi Y, Biswas KK, . Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-kappaB pathway inhibition. FEBS Lett 2006; 580: 613619. Crossref, Medline
19. Xu J, Wu HF, Ang ES, . NF-kappaB modulators in osteolytic bone diseases. Cytokine Growth Factor Rev 2009; 20: 717. Crossref, Medline

Vol 18, Issue 1, 2012

Recommended Citation

Curcumin modulates the immune response associated with LPS-induced periodontal disease in rats

Morgana R Guimarães1, Sabrina Garcia de Aquino1, Leila S Coimbra1, Luis C Spolidorio2, Keith L Kirkwood3, Carlos Rossa, Jr1Department of Diagnosis and Surgery, Faculdade de Odontologia de Araraquara, Univ Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Physiology and Pathology, Faculdade de Odontologia de Araraquara, Univ Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Craniofacial Biology and Center for Oral Health, College of Dental Medicine, Medical University of South Carolina (MUSC), Charleston, SC, USA

Innate Immunity

Vol 18, Issue 1, pp. 155 - 163

First published date: January-17-2011

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Morgana R Guimarães, Sabrina Garcia de Aquino, Leila S Coimbra, Luis C Spolidorio, Keith L Kirkwood, and Carlos Rossa, Jr
Innate Immunity 2011 18:1, 155-163

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