Curcumin is a plant-derived dietary spice ascribed various biological activities. Curcumin therapeutic applications have been studied in a variety of conditions, but not on periodontal disease. Periodontal disease is a chronic inflammatory condition initiated by an immune response to micro-organisms of the dental biofilm. Experimental periodontal disease was induced in rats by injecting LPS in the gingival tissues on the palatal aspect of upper first molars (30 µg LPS, 3 times/week for 2 weeks). Curcumin was administered to rats daily via oral gavage at 30 and 100 mg/kg body weight. Reverse transcriptase-qPCR and ELISA were used to determine the expression of IL-6, TNF-α and prostaglandin E2 synthase on the gingival tissues. The inflammatory status was evaluated by stereometric and descriptive analysis on hematoxylin/eosin-stained sections, whereas modulation of p38 MAPK and NK-κB signaling was assessed by Western blot. Curcumin effectively inhibited cytokine gene expression at mRNA and protein levels, but NF-κB was inhibited only with the lower dose of curcumin, whereas p38 MAPK activation was not affected. Curcumin produced a significant reduction on the inflammatory infiltrate and increased collagen content and fibroblastic cell numbers. Curcumin potently inhibits innate immune responses associated with periodontal disease, suggesting a therapeutic potential in this chronic inflammatory condition.

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Vol 18, Issue 1, 2012

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Curcumin modulates the immune response associated with LPS-induced periodontal disease in rats

Morgana R Guimarães1, Sabrina Garcia de Aquino1, Leila S Coimbra1, Luis C Spolidorio2, Keith L Kirkwood3, Carlos Rossa, Jr1Department of Diagnosis and Surgery, Faculdade de Odontologia de Araraquara, Univ Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Physiology and Pathology, Faculdade de Odontologia de Araraquara, Univ Estadual Paulista (UNESP), Araraquara, SP, BrazilDepartment of Craniofacial Biology and Center for Oral Health, College of Dental Medicine, Medical University of South Carolina (MUSC), Charleston, SC, USA


Innate Immunity

Vol 18, Issue 1, pp. 155 - 163

First published date: January-17-2011


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Morgana R Guimarães, Sabrina Garcia de Aquino, Leila S Coimbra, Luis C Spolidorio, Keith L Kirkwood, and Carlos Rossa, Jr
Innate Immunity 2011 18:1, 155-163

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