University at Buffalo - The State University of New York
Skip to Content
The timeline of metalloprotease events during oligofructose induced equine laminitis development. - PubMed - NCBI

Send to

Choose Destination
See comment in PubMed Commons below
Equine Vet J. 2012 Jan;44(1):88-93. doi: 10.1111/j.2042-3306.2011.00393.x. Epub 2011 Jun 23.

The timeline of metalloprotease events during oligofructose induced equine laminitis development.

Author information

The Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Australia.



The role of matrix metalloproteases (MMPs) and the timeline of proteolysis during laminitis development are incompletely understood.


To determine the temporal progression of selected MMPs and protease regulators during laminitis development.


Five clinically normal Standardbred horses received, via nasogastric intubation, an oligofructose (OF) bolus (10 g/kg bwt). Laminitis induction proceeded for 48 h followed by euthanasia. Lamellar biopsies were obtained prior to dosing and at intervals during the treatment period for analysis (12, 18, 24, 30 and 36 h and at 48 h following euthanasia). Tissue samples were analysed by real-time PCR, zymography and western blotting.


Activation of proMMP-2 occurs either simultaneously or at least 12 h following lamellar basement membrane (BM) damage, while no activation of proMMP-9 is seen during OF laminitis induction. Aggrecanase gene expression increased initially at 12-18 h post OF dosing, similar to BM changes. Gene expression of TIMP-2, a MMP regulator, decreases during laminitis development.


The MMP-2/MT1-MMP complex may not play a major role in initiating lamellar BM damage. Aggrecanase and TIMP-2 gene expression appear related to BM lamellar changes.


MMPs, historically thought to cause laminitis, do not appear to play an initiating role in the lamellar lesion. Other host derived proteases and degradation of alternative lamellar matrix components need to be considered.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center