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The Clinical Proteomic Technologies for Cancer | Details for Proliferating Cell Nuclear Antigen Peptide 1

Proliferating Cell Nuclear Antigen Peptide 1


Background

Catalog Number:

CPTC-PCNA-1

Target Antigen:

Proliferating Cell Nuclear Antigen Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

05/22/2014

Antigen Recognition(s):

peptide

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Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PCNA-1 Indirect ELISA (peptide)

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

Background

NCI Identification Number:

00035

Antigen Name:

Proliferating Cell Nuclear Antigen Peptide 1

CPTC Name:

CPTC-PCNA Peptide 1

Keywords:

Cyclin; DNA Polymerase Delta Auxiliary Protein

Function:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Chromosomal Localization:

20pter-p12

Expression System:

NCBI Accession Number:

NP_002583.1

Swiss Prot Accession Number:

P12004

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LVQGSILK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

880

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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