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PubMed Central, Figure 5: Nat Cell Biol. 2014 Oct; 16(10): 1004–13. Published online 2014 Sep 21. doi:  10.1038/ncb3040
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Nat Cell Biol. Author manuscript; available in PMC 2015 Apr 1.
Published in final edited form as:
Nat Cell Biol. 2014 Oct; 16(10): 1004–13.
Published online 2014 Sep 21. doi:  10.1038/ncb3040

Figure 5

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ΔNp63 expression positively correlates with FZD7 expression and Wnt signaling in human breast cancer

(a) ΔNp63 expression in clinical samples of basal and luminal type human tumors. Box represents 75th, 50th and 25th percentile of the values. Top and bottom lines represent maximum and minimal data points within 1.5x IQ (inter quarter) range, respectively (n = 286 tumors). (b) Scatter plot showing correlation between ΔNp63 and FZD7 expression in breast tumors from the TCGA-BRCA-RNAseqV2 dataset46 (n = 1000 tumors). (c) IHC analysis of ΔNp63 and FZD7 expression. (d) Relative expression of Wnt luciferase reporter in MDA-MB-231 cells after lentiviral transduction of a Wnt reporter (7TFC), together with indicated shRNAs. (e) Western blot of ΔNp63, TAp63 and FZD7 expression in MDA-MB-231 and SUM-1315 cells. (f, g) Western blot of ΔNp63 and FZD7 expression in MDA-MB-231 and SUM-1315 cells after transduction with indicated shRNAs. (h) Fold change of TAp63 mRNA level in MDA-MB-231 cells and SUM-1315 cells respectively, after transduction with control and TAp63 shRNA. (i) Relative expression of Wnt luciferase reporter in MDA-MB-231 cells after lentiviral transduction of a Wnt reporter (7TFC), together with indicated shRNAs. In d, h and i, n = 3 samples, data represents mean ± s.d. (j) Tumor incidence of MDA-MB-231 cells expressing indicated shRNAs. (k) Tumor incidence of SUM-1315 cells expressing indicated shRNAs. In j and k, n= number of mammary fat pad injections as indicated in the table. P value was obtained by Pearson’s Chi-squared test using ELDA software. (l) Quantification of tumorsphere formed by control, p63-KD and Fzd7-KD tumors (10,000 cells) from PDX-2 (HCI002)48. (m) Representative images of tumorspheres from l. (n–p) Quantification of tumorsphere formed by control, ΔNp63 KD and TAp63 KD cells (10,000 cells) from PDX-2 tumors (HCI002) (n–o) and PDX-3 tumors (HCI009) (p). In l, n–p, n = 4 tumors; data represents mean ± s.d. *p < 0.05 by Student’s t test. Size bar, 40 μm in c and m respectively. Uncropped images of blots are shown in Supplementary Fig. 9.

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