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Assay Portal | Office of Cancer Clinical Proteomics Research

Overview Data source: UniProt

Official Gene Symbol Other Aliases
APOE N/A
Sequence Length (AA) Molecular Weight (Da)
317 36154
Protein Name
Apolipoprotein E
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKVLWAALLV TFLAGCQAKV EQAVETEPEP ELRQQTEWQS GQRWELALGR
60 70 80 90 100
FWDYLRWVQT LSEQVQEELL SSQVTQELRA LMDETMKELK AYKSELEEQL
110 120 130 140 150
TPVAEETRAR LSKELQAAQA RLGADMEDVC GRLVQYRGEV QAMLGQSTEE
160 170 180 190 200
LRVRLASHLR KLRKRLLRDA DDLQKRLAVY QAGAREGAER GLSAIRERLG
210 220 230 240 250
PLVEQGRVRA ATVGSLAGQP LQERAQAWGE RLRARMEEMG SRTRDRLDEV
260 270 280 290 300
KEQVAEVRAK LEEQAQQIRL QAEAFQARLK SWFEPLVEDM QRQWAGLVEK
310 317
VQAAVGTSAA PVPSDNH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1087 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOE
Peptide Sequence
LGPLVEQGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
199
Peptide End
207
CPTAC ID
non-CPTAC-1087
Peptide Molecular Mass
967.5451
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 8.9 3.9 4.2 10.7 7.8 4.9 13.9 8.7 6.5 15 15 15
y8 (2+) 12.7 4.9 3.4 12.2 7.8 4.9 17.6 9.2 6 15 15 15
y4 (1+) 6.1 3.6 2.8 9.9 6.4 4.6 11.6 7.3 5.4 15 15 15
y5 (1+) 7.1 3.9 6.1 11.5 5.5 5.7 13.5 6.7 8.3 15 15 15
y6 (1+) 9.2 5.9 4.1 14.1 5.9 4.5 16.8 8.3 6.1 15 15 15
sum 5.6 2.4 2.5 8.8 6.2 3.3 10.4 6.6 4.1 15 15 15

Additional Resources and Comments