University at Buffalo - The State University of New York
Skip to Content
Assay Portal | Office of Cancer Clinical Proteomics Research

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINE1 PAI1,PLANH1
Sequence Length (AA) Molecular Weight (Da)
402 45060
Protein Name
Plasminogen activator inhibitor 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQMSPALTCL VLGLALVFGE GSAVHHPPSY VAHLASDFGV RVFQQVAQAS
60 70 80 90 100
KDRNVVFSPY GVASVLAMLQ LTTGGETQQQ IQAAMGFKID DKGMAPALRH
110 120 130 140 150
LYKELMGPWN KDEISTTDAI FVQRDLKLVQ GFMPHFFRLF RSTVKQVDFS
160 170 180 190 200
EVERARFIIN DWVKTHTKGM ISNLLGKGAV DQLTRLVLVN ALYFNGQWKT
210 220 230 240 250
PFPDSSTHRR LFHKSDGSTV SVPMMAQTNK FNYTEFTTPD GHYYDILELP
260 270 280 290 300
YHGDTLSMFI AAPYEKEVPL SALTNILSAQ LISHWKGNMT RLPRLLVLPK
310 320 330 340 350
FSLETEVDLR KPLENLGMTD MFRQFQADFT SLSDQEPLHV AQALQKVKIE
360 370 380 390 400
VNESGTVASS STAVIVSARM APEEIIMDRP FLFVVRHNPT GTVLFMGQVM
402
EP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



loading

Loader

Assay Details for non-CPTAC-1150 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINE1
Peptide Sequence
VFQQVAQASK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
42
Peptide End
51
CPTAC ID
non-CPTAC-1150
Peptide Molecular Mass
1,104.5928
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.1 5.1 8.5 15.6 8.3 11 19.7 9.7 13.9 14 15 15
y8 (2+) 17.5 11.7 9.6 17.1 10.6 12.7 24.5 15.8 15.9 15 15 15
y5 (1+) 9.7 10.6 7.4 8.9 13.6 8.2 13.2 17.2 11 15 15 15
y7 (1+) 28.7 10.3 7.5 23.1 12.4 8.8 36.8 16.1 11.6 15 15 15
y8 (1+) 16.9 11 7 16.9 10.5 7.4 23.9 15.2 10.2 15 15 15
sum 8.6 5.3 4.8 7.6 5.9 4.5 11.5 7.9 6.6 15 15 15

Additional Resources and Comments