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17308176[PMID] - PMC - NCBI

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1.
FIG. 2.

FIG. 2. From: Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached Bacteria .

Schematic drawing of the bacterial overlay method modified for detection of surface-adsorbed microorganisms. Attached biotinylated bacterial organisms were detected by fluorescein-tagged avidin-D. The fluorescence signal emitted was recorded by a fluorescence scanner.

Rainer Müller, et al. Appl Environ Microbiol. 2007 Apr;73(8):2653-2660.
2.
FIG. 3.

FIG. 3. From: Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached Bacteria .

Effect of protein precoating on advancing water contact angles of the chemically modified silicon wafers. FN, fibronectin; OX, hydroxyl group created by oxidation.

Rainer Müller, et al. Appl Environ Microbiol. 2007 Apr;73(8):2653-2660.
3.
FIG. 1.

FIG. 1. From: Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached Bacteria .

Properties of binding of the bacterial strains employed in this study to the proteins and biological fluids used for surface precoating. Proteins were immobilized as dots on nitrocellulose membranes. Binding of fluorescence-labeled bacteria to the immobilized proteins was measured by a fluorescence scanner and is visualized by dark spots.

Rainer Müller, et al. Appl Environ Microbiol. 2007 Apr;73(8):2653-2660.
4.
FIG. 4.

FIG. 4. From: Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached Bacteria .

Bacterial attachment detected by SEM and agar replica plating (imprint) in comparison to that detected by the fluorescence-based bacterial overlay technique. The comparison of the three techniques is exemplarily shown for S. gordonii DL1 on differently modified smooth wafers without protein precoating. In the images of the agar imprint, whitish areas are indicative of colony formation. In the images of the bacterial overlay, dark areas are indicative of fluorescence from attached bacteria. OX, hydroxyl group created by oxidation.

Rainer Müller, et al. Appl Environ Microbiol. 2007 Apr;73(8):2653-2660.
5.
FIG. 6.

FIG. 6. From: Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached Bacteria .

Numbers of bacteria attached on silicon wafers, depending on surface roughness, chemical surface modification, and preadsorbed protein layers. Numbers were calculated by comparison of the signal intensities from wafer surfaces with the standard curves for each bacterium. (A) S. gordonii on smooth surfaces; (B) S. gordonii on rough surfaces; (C) S. mitis on smooth surfaces; (D) S. mitis on rough surfaces; (E) S. aureus on smooth surfaces; (F) S. aureus on rough surfaces. OX, hydroxyl group created by oxidation.

Rainer Müller, et al. Appl Environ Microbiol. 2007 Apr;73(8):2653-2660.
6.
FIG. 5.

FIG. 5. From: Fluorescence-Based Bacterial Overlay Method for Simultaneous In Situ Quantification of Surface-Attached Bacteria .

Quantification of attached bacteria by bacterial overlay on differently modified silicon wafers. (A) Image of fluorescent signals (dark areas) after attachment of S. gordonii DL1 to surfaces of various morphologies and surface chemistries. (The upper left panel, right column, is identical to the overlay image shown in Fig. .) (B) Signals obtained after fluorescence labeling of serial twofold dilutions of the three different bacterial strains immobilized on nitrocellulose membranes. (C) Standard curve for quantification of S. gordonii DL1 after densitometric analysis of signals obtained from the standard dilutions. FN, fibronectin; OX, hydroxyl group created by oxidation.

Rainer Müller, et al. Appl Environ Microbiol. 2007 Apr;73(8):2653-2660.

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