University at Buffalo - The State University of New York
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Protein Production and Crystallization - Jacobs School of Medicine and Biomedical Sciences - University at Buffalo
University at Buffalo - The State University of New York
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Core Facilities

Protein Production and Crystallization

Joseph Luft at the Protein Production and Crystallization facility

Joseph Luft at the Protein Production and Crystallization facility.

Services

Our facility offers instrumentation and the technical expertise required to express and purify milligram quantities of soluble and membrane proteins for biophysical and structural characterization.

At the core of our facility is a high-throughput screening laboratory for use in the identification of initial crystallization leads for a target protein.

The laboratory uses two sets of 1536 crystallization cocktails for soluble and membrane proteins, set up using the microbatch-under-oil crystallization technique. We monitor experiments using digital recordings of the results.

Full laboratory facilities are available for the identification of protein crystals, optimization of crystallization leads and structure determination using X-ray crystallography.

Instrumentation

  • Dedicated tissue culture room with two biosafety hoods, eight incubators, fourteen stir plates, controlled O2/air flow, and the necessary spinner flasks for large-scale production of proteins in insect cells
  • Two 10L New Brunswick BIOFLO 310 fermentor/bioreactors for large-scale production of proteins in E. coli and S. cerevisiae expression hosts
  • Microfluidics Corp. M-110EH Microfluidizer utilized for large-scale cell disruptions
  • Waters Breeze-2 HPLC system, equipped with an auto sampler and high sensitivity detectors in series (refractive index, UV/Vis, viscosity, and right angle light scattering) to perform molecular weight, molecular size, and intrinsic viscosity of protein-protein and protein-detergent complexes using size-exclusion chromatography
  • Protein Solutions Dynapro temperature-controlled dynamic light scattering instrument and a Wyatt Technology Corporation mini DAWN triple-angle light scattering detector utilized to evaluate sample homogeneity
  • Brookfield programmable DV-II viscometer, a Kruss K-11 tensiometer and a Wescor 5520 Vapro Vapor Pressure Osmometer to measure specific physical and chemical properties of solutions
  • JANSi UVEX UV microscope and second order non-linear imaging of chiral crystals (SONICC) imaging system coupled with UV-Two Photon Excited Fluorescence (UV-TPEF) to aid in the identification of protein crystals that are not visually observable to the naked eye

Location

Hauptman Woodward Medical Research Institute
Buffalo Niagara Medical Campus
700 Ellicott Street
Buffalo, N.Y.
(716) 898-8600

Contact Information

Malkowski, Michael

Michael Malkowski, PhD

Professor and Chair

Department of Structural Biology, Hauptman-Woodward Institute, 700 Ellicott Street Buffalo, NY 14203

Phone: 716-898-8624; Fax: 716-898-8660

Email: mgm22@buffalo.edu

Snell, Edward

Edward Snell, PhD

Senior Scientist, CEO of Hauptman-Woodward Medical Institute

Hauptman-Woodward Institute, 700 Ellicott St. Buffalo, NY 14203

Phone: (716) 898-8631; Fax: (716) 898-8660

Email: esnell@buffalo.edu