University at Buffalo - The State University of New York
Skip to Content
Assay Portal | Office of Cancer Clinical Proteomics Research

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CD3EAP ASE1,CAST,PAF49
Sequence Length (AA) Molecular Weight (Da)
510 54986
Protein Name
DNA-directed RNA polymerase I subunit RPA34
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEEPQAGDAA RFSCPPNFTA KPPASESPRF SLEALTGPDT ELWLIQAPAD
60 70 80 90 100
FAPECFNGRH VPLSGSQIVK GKLAGKRHRY RVLSSCPQAG EATLLAPSTE
110 120 130 140 150
AGGGLTCASA PQGTLRILEG PQQSLSGSPL QPIPASPPPQ IPPGLRPRFC
160 170 180 190 200
AFGGNPPVTG PRSALAPNLL TSGKKKKEMQ VTEAPVTQEA VNGHGALEVD
210 220 230 240 250
MALGSPEMDV RKKKKKKNQQ LKEPEAAGPV GTEPTVETLE PLGVLFPSTT
260 270 280 290 300
KKRKKPKGKE TFEPEDKTVK QEQINTEPLE DTVLSPTKKR KRQKGTEGME
310 320 330 340 350
PEEGVTVESQ PQVKVEPLEE AIPLPPTKKR KKEKGQMAMM EPGTEAMEPV
360 370 380 390 400
EPEMKPLESP GGTMAPQQPE GAKPQAQAAL AAPKKKTKKE KQQDATVEPE
410 420 430 440 450
TEVVGPELPD DLEPQAAPTS TKKKKKKKER GHTVTEPIQP LEPELPGEGQ
460 470 480 490 500
PEARATPGST KKRKKQSQES RMPETVPQEE MPGPPLNSES GEEAPTGRDK
510
KRKQQQQQPV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



loading

Loader

Assay Details for CPTAC-920 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD3EAP
Peptide Modified Sequence
FSC[+57.0]PPNFTAKPPASES[+80.0]PR
Modification Type
Carbamidomethyl Cysteine, Phospho (ST)
Protein - Site of Modification
14, 27
Peptide - Site of Modification
3, 16
Peptide Start
12
Peptide End
29
CPTAC ID
CPTAC-920
Peptide Molecular Mass
2,068.9078
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y14 (2+) 37 25.8 31.4 43.5 26.2 48 57.1 36.8 57.4 12 24 15
y15 (2+) 42.7 25.6 24.1 32.9 31.9 32.8 53.9 40.9 40.7 12 24 15
y8 (1+) 45.9 36.5 65.3 26.7 42.1 50.8 53.1 55.7 82.7 12 24 15
sum 35.3 21.9 24 25.8 25.5 25.8 43.7 33.6 35.2 12 24 15

Additional Resources and Comments


Assay Details for CPTAC-921 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD3EAP
Peptide Modified Sequence
QEQINTEPLEDTVLS[+80.0]PTK
Modification Type
Phospho (ST)
Protein - Site of Modification
285
Peptide - Site of Modification
15
Peptide Start
271
Peptide End
288
CPTAC ID
CPTAC-921
Peptide Molecular Mass
2,120.9879
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 23.3 11.2 22.3 39.2 19.7 24 45.6 22.7 32.8 12 20 13
y11 (2+) 22.9 23.5 16.9 24.8 33.6 28.7 33.8 41 33.3 12 20 13
y4 (1+) 39 24.9 36.9 35.5 26.5 45.7 52.7 36.4 58.7 12 20 13
sum 15.6 13.7 16.8 20.7 21.6 27.2 25.9 25.6 32 12 20 13

Additional Resources and Comments