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Draining gut and liver lymph node T-cell hematopoiesis. (a–f) 12 week-old male SPF & GF mice were euthanized; Mesenteric lymph node (MLN) and liver lymph node (LLN) cells were isolated and stained for flow cytometric analysis (n = 4/gp). (a) % CD3+CD4+CD8− (helper) T-cells, (b) % CD3+CD4−CD8+ (cytotoxic) T-cells, and (c) % CD3+CD4−CD8−TCRγδ+ (gamma delta) T-cells are expressed relative to total lymph node cells. (d) % CD4+CD25+FOXP3+ (TREG) cells, (e) % CD4+CD196+RORγt+ (TH17) cells, and (f) % CD4+CD183+T-bet+ (TH1) cells are expressed relative to CD4+ cells. Data are reported as mean ± SEM. **p < 0.01 vs. SPF; ***p < 0.001 vs. SPF.