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1.
Figure 1

Figure 1. Observed concentration of IL-1β in serum sample spiked 1∶20 with highest calibrator and then diluted and analyzed in replicate.. From: Performance of Multiplex Cytokine Assays in Serum and Saliva among Community-Dwelling Postmenopausal Women.

Initial 1∶2 dilution causes a 3 fold increase in observed concentration followed by 50% decreases with each successive serial dilution.

Richard W. Browne, et al. PLoS One. 2013;8(4):e59498.
2.
Figure 2

Figure 2. Empirical estimation of the lower limit of quantification (LLOQ) for matrix metalloproteinase 1 (MMP-1) and MMP-2.. From: Performance of Multiplex Cytokine Assays in Serum and Saliva among Community-Dwelling Postmenopausal Women.

The x-axis is analyte concentration and the y-axis is the coefficient of variation of six replicate measurements of authentic serum spiked with analyte by standard additions methodology.

Richard W. Browne, et al. PLoS One. 2013;8(4):e59498.

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