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Transcription and genetic analyses of a putative N-acetylmuramyl-L-alanine amidase in Borrelia burgdorferi. - PubMed - NCBI
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FEMS Microbiol Lett. 2009 Jan;290(2):164-73. doi: 10.1111/j.1574-6968.2008.01416.x. Epub 2008 Nov 18.

Transcription and genetic analyses of a putative N-acetylmuramyl-L-alanine amidase in Borrelia burgdorferi.

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1
Department of Oral Biology, State University of New York at Buffalo, NY 14214-3092, USA.

Abstract

In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb0665 and bb0667) are cotranscribed with the motility and chemotaxis genes. This newly identified operon is termed as pami. Sequence and primer extension analyses showed that pami was regulated by a sigma(70)-like promoter, which is designated as P(ami). Transcriptional analysis using a gene encoding green fluorescence protein as a reporter demonstrated that P(ami) functions in both Escherichia coli and B. burgdorferi. Genetic studies showed that the Deltabb0666 mutant grows in long chains of unseparated cells, whose phenotype is similar to its counterparts in E. coli. Taken together, these results demonstrate that bb0666 is a homolog of MurNac-LAAs that contributes to the cell division of B. burgdorferi.

PMID:
19025570
PMCID:
PMC2927206
DOI:
10.1111/j.1574-6968.2008.01416.x
[Indexed for MEDLINE]
Free PMC Article
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