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Growth of T. forsythia biofilm on glycoprotein surfaces. (a) Biofilm growth was assessed for the wild-type strain in the absence (black bars) or presence of oseltamivir (grey bars). Biofilms were set up in triplicate and wells were supplemented with 6 mM sialic acid (sialic) or 6 mM sialyl-lactose (sia-lac), or coated with 6 mM mucin, fetuin and asialofetuin (as-fet), 2 µg human serum ml−1 (serum) or saliva, with and without the addition of 10 mM oseltamivir to the TSB medium, as indicated at the time of inoculation. Glycoproteins were coated on the 96-well plate overnight at 4 °C and washed before inoculation with T. forsythia to a final OD600 of 0.05. Data are the mean number of cells with sds after harvesting from wells after 5 days. Differences between mean values in this experiment were evaluated by t test, with P<0.05 being taken as the level of significance (*statistically significant). (b) Biofilm growth was compared as above between the wild-type (wt) strain and the ΔnanH mutant strain of T. forsythia (ΔnanH).