[HTML][HTML] Development of an inducible gene expression system for primary murine keratinocytes

P Nagarajan, S Sinha - Journal of dermatological science, 2008 - Elsevier
BACKGROUND: The tetracycline (Tet) responsive system is a valuable tool that is routinely
used in a wide variety of mammalian cells for regulatable expression of gene products.
However, technical difficulties such as harsh selection conditions and extensive screening
processes to identify suitably responsive clones limit the generation of stable cell lines.
Hence, application of this system in mammalian cells with relatively slow growth rates and¬†…