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Assay Portal | Office of Cancer Clinical Proteomics Research

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CAP1 CAP
Sequence Length (AA) Molecular Weight (Da)
475 51901
Protein Name
Adenylyl cyclase-associated protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MADMQNLVER LERAVGRLEA VSHTSDMHRG YADSPSKAGA APYVQAFDSL
60 70 80 90 100
LAGPVAEYLK ISKEIGGDVQ KHAEMVHTGL KLERALLVTA SQCQQPAENK
110 120 130 140 150
LSDLLAPISE QIKEVITFRE KNRGSKLFNH LSAVSESIQA LGWVAMAPKP
160 170 180 190 200
GPYVKEMNDA AMFYTNRVLK EYKDVDKKHV DWVKAYLSIW TELQAYIKEF
210 220 230 240 250
HTTGLAWSKT GPVAKELSGL PSGPSAGSCP PPPPPCPPPP PVSTISCSYE
260 270 280 290 300
SASRSSLFAQ INQGESITHA LKHVSDDMKT HKNPALKAQS GPVRSGPKPF
310 320 330 340 350
SAPKPQTSPS PKRATKKEPA VLELEGKKWR VENQENVSNL VIEDTELKQV
360 370 380 390 400
AYIYKCVNTT LQIKGKINSI TVDNCKKLGL VFDDVVGIVE IINSKDVKVQ
410 420 430 440 450
VMGKVPTISI NKTDGCHAYL SKNSLDCEIV SAKSSEMNVL IPTEGGDFNE
460 470 475
FPVPEQFKTL WNGQKLVTTV TEIAG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-325 Collapse assay details

Data source: Panorama

Official Gene Symbol
CAP1
Peptide Sequence
LSDLLAPISEQIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
101
Peptide End
113
CPTAC ID
CPTAC-325
Peptide Molecular Mass
1,425.8079
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 16.4 8.1 4.4 26.8 11.7 7.3 31.4 14.2 8.5 15 25 15
y8 (1+) 10.9 7 6.1 20.8 9.7 8.9 23.5 12 10.8 15 25 15
y7 (1+) 9 5.8 3.6 19.4 7.6 7.5 21.4 9.6 8.3 15 25 15
sum 8.1 5.9 3.6 19 8.3 6.3 20.7 10.2 7.3 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-326 Collapse assay details

Data source: Panorama

Official Gene Symbol
CAP1
Peptide Sequence
VENQENVSNLVIEDTELK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
331
Peptide End
348
CPTAC ID
CPTAC-326
Peptide Molecular Mass
2,072.0273
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 14.2 5.8 7.4 17.2 7.5 10.4 22.3 9.5 12.8 25 15 15
y7 (1+) 9.2 4.3 4.9 12 5.6 6.8 15.1 7.1 8.4 25 15 15
y5 (1+) 18.5 11.9 10.9 20.7 13.3 28.9 27.8 17.8 30.9 25 15 15
sum 7.7 3.6 3.1 10.3 4.3 6.5 12.9 5.6 7.2 25 15 15

Additional Resources and Comments