Editor: Robert Burne
Transcription and genetic analyses of a putative N-acetylmuramyl-l-alanine amidase in Borrelia burgdorferi
Version of Record online: 19 NOV 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 290, Issue 2, pages 164–173, January 2009
How to Cite
Yang, Y. and Li, C. (2009), Transcription and genetic analyses of a putative N-acetylmuramyl-l-alanine amidase in Borrelia burgdorferi. FEMS Microbiology Letters, 290: 164–173. doi: 10.1111/j.1574-6968.2008.01416.x
- Issue online: 8 DEC 2008
- Version of Record online: 19 NOV 2008
- Received 28 August 2008; accepted 15 October 2008.First published online 19 November 2008.
- lyme disease;
- Borrelia burgdorferi;
- N-acetylmuramyl-l-alanine amidase
In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb0665 and bb0667) are cotranscribed with the motility and chemotaxis genes. This newly identified operon is termed as pami. Sequence and primer extension analyses showed that pami was regulated by a σ70-like promoter, which is designated as Pami. Transcriptional analysis using a gene encoding green fluorescence protein as a reporter demonstrated that Pami functions in both Escherichia coli and B. burgdorferi. Genetic studies showed that the Δbb0666 mutant grows in long chains of unseparated cells, whose phenotype is similar to its counterparts in E. coli. Taken together, these results demonstrate that bb0666 is a homolog of MurNac-LAAs that contributes to the cell division of B. burgdorferi.