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PubMed Central, Figure 1: Nat Cell Biol. 2014 Oct; 16(10): 1004–13. Published online 2014 Sep 21. doi:  10.1038/ncb3040
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
PMC full text:
Nat Cell Biol. Author manuscript; available in PMC 2015 Apr 1.
Published in final edited form as:
Nat Cell Biol. 2014 Oct; 16(10): 1004–13.
Published online 2014 Sep 21. doi:  10.1038/ncb3040

Figure 1

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ΔNp63 is enriched in MaSCs and promote MaSC activity

(a) Heat map of differential expression of 26 transcriptional regulators, including p63 (arrow), in different mouse MEC subpopulations (upper panel). qRT-PCR analysis of ΔNp63 and TAp63 mRNAs in MECs (lower panel) (n = 3 samples; data represents mean ± s.d.). qRT-PCR values were normalized to Gapdh. (b) Immunoblot of ΔNp63 expression in different mouse MEC subpopulations. (c) Immunohistochemical analysis of ΔNp63 and TAp63 expression in the terminal end buds (TEBs) and ducts of mammary gland serial sections. (d) qRT-PCR analysis of ΔNp63 and TAp63 mRNAs in normal human mammary epithelium (left panel, n = 3 samples; data represents mean ± s.d.) and CD49fhiEpCAMneg/low MaSC-enriched population63 (right panel, n = 3 samples; data represents mean ± s.d.). qRT-PCR values were normalized to GAPDH. *p < 0.05 by Student’s t test. (e) Strategy of lentiviral transduction of different MEC subpopulations. (f) MaSC-enriched LinCD24+CD29hi (P4) population was isolated and transduced with indicated lentivirus constructs and transplanted into cleared fat pads (1000 cells). (g) 1000 luminal cells (LinCD24+CD29lo) (P5) were transduced with indicated lentivirus constructs and injected to cleared fat pad. Representative mammary outgrowths shown in f and g. In pie graph below representative images, each circle represents one mammary gland, with blackened area representing the degree of gland filling with outgrowth. ● 80–100%, An external file that holds a picture, illustration, etc.
Object name is nihms622121ig1.jpg 30–80%, ◔ 0–30% and no reconstitution. (h, i) Pie graph (h) and representative fluorescence images (i) of the mammary fat pads after competitive reconstitution assay. (j) Representative images of 3-D matrigel colonies of P4 population from GFP and dsRED MECs mixed equally. (k) Number of colonies formed in three generations in 3-D matrigel assay (n = 3 samples; data represents mean ± s.d.). (l) Table showing reconstitution rate of the mammary gland over successive generations after transplantation of 10,000 unsorted MECs transduced with control or ΔNp63 expressing lentiviruses. *p < 0.05, **p < 0.01 by Student’s t test. Size bar, 40 μm in c, 1 mm in f and g, 2mm in i, and 3mm in j. Uncropped images of blots are shown in Supplementary Fig. 9.

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