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PubMed Central, Figure 6: Nat Cell Biol. 2014 Oct; 16(10): 1004–13. Published online 2014 Sep 21. doi:  10.1038/ncb3040
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
PMC full text:
Nat Cell Biol. Author manuscript; available in PMC 2015 Apr 1.
Published in final edited form as:
Nat Cell Biol. 2014 Oct; 16(10): 1004–13.
Published online 2014 Sep 21. doi:  10.1038/ncb3040

Figure 6

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Loss of ΔNp63 attenuates tumor initiation in basal-like MMTV-Wnt1 tumors

(a) Fold change of ΔNp63 mRNA level in primary MMTV-Wnt1 tumor MECs after transduction with indicated shRNAs (n = 3 tumors). (b) Quantification of tumorsphere numbers from MMTV-Wnt1 tumor cells transduced with indicated shRNAs (n = 3 tumors). (c) Kaplan-Meier curve of tumor-free mammary gland incidence using MMTV-Wnt1 tumor MECs (10,000 cells) transudced with indicated shRNAs. Log rank test was used for statistical analysis (n = 8 mice). (d) Average tumor volume at end point control (n = 8 tumors) and p63 KD1 (n = 7 tumors). (e) Tumor incidence of MMTV-Wnt1 tumor cells expressing the indicated shRNAs (). (f) Representative FACS profile of primary tumors. (g) Quantification of percentage of CD45CD24+Thy-1+ cells in control and p63-KD Wnt1 tumor cells as shown in f (n = 10 tumors). (h) qRT-PCR analysis of Fzd7 and p63 expression in control or p63-KD Wnt1 tumor MECs (n = 3 samples). (i) Western blot of ΔNp63 in control and ΔNp63-KD MMTV-Wnt1 tumor cells. (j) Fold change of TAp63 mRNA level in MMTV-Wnt1 tumor cells after TAp63 KD (n = 3 tumors). (k and l) Quantification of tumorspheres formed by 10,000 MMTV-Wnt1 tumor cells transduced with indicated shRNAs (n = 3 tumors in k and n = 4 tumors in l). (m) Kaplan-Meier curve analysis of tumor-free mammary glands after injection of mice using control or Fzd7-KD MMTV-Wnt1 tumor MECs (10,000 cells). n = 8 mice. Log rank test was used for statistical analysis. (n) Average tumor volume at end point (n = 6 tumors). (o) Tumor incidence of MMTV-Wnt1 tumor cells expressing the indicated shRNAs. In this figure, bar graph data represent mean ± s.d. *p < 0.05 by Student’s t test in a, b, h, j, k and l. *p < 0.05 by Mann Whitney test in d, g and n. In e and o, n= number of mammary fat pad injections as indicated in the table. p value was obtained by Pearson’s Chi-squared test using ELDA software. Uncropped images of blots are shown in Supplementary Fig. 9.

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