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PubMed Central, Figure 2: Nat Cell Biol. 2012 Nov; 14(11): 1212–1222. Published online 2012 Oct 21. doi:  10.1038/ncb2607
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
PMC full text:
Nat Cell Biol. Author manuscript; available in PMC 2013 May 1.
Published in final edited form as:
Nat Cell Biol. 2012 Nov; 14(11): 1212–1222.
Published online 2012 Oct 21. doi:  10.1038/ncb2607

Figure 2

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Loss of Elf5 leads to increased EMT gene expression programs

(a) Heat map representation of microarray data displaying the expression of several key EMT-related genes in wild type and Elf5-KO mammary glands on lactation day 1. (b) GSEA data showing the enrichment of four published EMT gene signatures38-41 in Elf5-KO mammary glands as compared to wild type glands. NES: normalized enrichment score. (c, e) Western blot analyses of HA-Elf5 and E-cadherin protein levels in NMuMG cells transduced with vector (control) or HA-Elf5. Uncropped images of blots are shown in Supplementary Fig. S9. (d) Phase contrast and E-cadherin immunofluorescence images of NMuMG (control) or NMuMG-Elf5 cells undergoing TGFβ-induced EMT when cultured in low cell density. Experiments using cells cultured in high density are shown in Supplementary Fig. S1i. Size bar = 100 μm for brightfield and 40 μm for E-cadherin. (f) qRT-PCR analysis of expression of EMT-related genes in NMuMG (control) or NMuMG-Elf5 cells, with or without TGFβ treatment. Real time PCR values were normalized to the housekeeping gene Gapdh. Experiments were performed three times, each with qRT-PCR in technical duplicate, and data presented as the mean ± SD. * p < 0.05 by Student’s t-test.

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