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Development of a Modified Gentamicin Resistance Cassette for Genetic Manipulation of the Oral Spirochete Treponema denticola

Development of a Modified Gentamicin Resistance Cassette for Genetic Manipulation of the Oral Spirochete Treponema denticola

  1. Chunhao Lia
  1. aDepartment of Oral Biology, State University of New York at Buffalo, Buffalo, New York, USA
  2. bDepartment of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, Michigan, USA

ABSTRACT

Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola.

FOOTNOTES

    • Received 4 November 2011.
    • Accepted 31 December 2011.
  • Address correspondence to Chunhao Li, cli9{at}buffalo.edu.
  • Published ahead of print 13 January 2012