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ΔNp63 can induce de novo expression of K5 and K14 in single-layered lung epithelia.
A) Schematic depicting the mating scheme used to generate transgenic animals expressing the HA-ΔNp63 transgene in single-layered lung epithelium using the surfactant protein C (SPC) promoter in a tetracycline inducible fashion. B) Gross morphology of E18.5 lung tissue sections stained with H&E from control animals and bi-transgenic animals (ΔNp63αBG and ΔNp63βBG). Bi-transgenic animals exhibit squamous metaplasia. Scale bar: 50 µm. C) Lung tissue sections from E18.5 stained with antibodies detecting HA epitope tag (green) and K5 or K14 (red) reveal expression of the transgene in bi-transgenic animals. Dapi staining is shown in blue. Tissues from bi-transgenic animals demonstrate induction of K5 and K14 expression (red staining) as compared to control littermates.