University at Buffalo - The State University of New York
Skip to Content
Assay Portal | Office of Cancer Clinical Proteomics Research

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP2K3 MEK3,MKK3,PRKMK3,SKK2
Sequence Length (AA) Molecular Weight (Da)
347 39318
Protein Name
Dual specificity mitogen-activated protein kinase kinase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MESPASSQPA SMPQSKGKSK RKKDLRISCM SKPPAPNPTP PRNLDSRTFI
60 70 80 90 100
TIGDRNFEVE ADDLVTISEL GRGAYGVVEK VRHAQSGTIM AVKRIRATVN
110 120 130 140 150
SQEQKRLLMD LDINMRTVDC FYTVTFYGAL FREGDVWICM ELMDTSLDKF
160 170 180 190 200
YRKVLDKNMT IPEDILGEIA VSIVRALEHL HSKLSVIHRD VKPSNVLINK
210 220 230 240 250
EGHVKMCDFG ISGYLVDSVA KTMDAGCKPY MAPERINPEL NQKGYNVKSD
260 270 280 290 300
VWSLGITMIE MAILRFPYES WGTPFQQLKQ VVEEPSPQLP ADRFSPEFVD
310 320 330 340 347
FTAQCLRKNP AERMSYLELM EHPFFTLHKT KKTDIAAFVK EILGEDS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



loading

Loader

Assay Details for CPTAC-814 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K3
Peptide Modified Sequence
FSPEFVDFTAQC[+57.0]LR
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
305
Peptide - Site of Modification
12
Peptide Start
294
Peptide End
307
CPTAC ID
CPTAC-814
Peptide Molecular Mass
1,715.7977
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 40.5 20.7 15.9 41.9 21.3 15.3 58.3 29.7 22.1 15 15 15
y6 (1+) 22.7 14.5 6 21.4 16.9 10.4 31.2 22.3 12 15 15 15
y7 (1+) 14.8 12.1 7.1 16.2 13 6.8 21.9 17.8 9.8 15 15 15
y8 (1+) 15.8 12.5 8.4 15.4 11.7 8.3 22.1 17.1 11.8 15 15 15
y9 (1+) 10.3 8.9 6.7 13.4 8.8 7.5 16.9 12.5 10.1 15 15 15
y10 (1+) 21.5 8 6.2 25.3 13.8 6.7 33.2 16 9.1 15 15 15
y11 (1+) 27.8 22 15.5 35.8 26 20 45.3 34.1 25.3 15 15 15
sum 7.5 5 3.1 7 6.7 3.1 10.3 8.4 4.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-815 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K3
Peptide Sequence
QVVEEPSPQLPADR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
280
Peptide End
293
CPTAC ID
CPTAC-815
Peptide Molecular Mass
1,563.7893
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 11.2 4.9 7.8 13.6 7.1 5.7 17.6 8.6 9.7 15 15 15
y5 (1+) 17.8 10.3 14.5 16.6 12.5 11.4 24.3 16.2 18.4 15 15 15
y7 (1+) 11.5 7.5 10.4 10.5 5.4 8.4 15.6 9.2 13.4 15 15 15
y8 (1+) 28.8 12.7 14.2 32.6 14.3 15 43.5 19.1 20.7 15 15 15
y9 (1+) 9.1 3.2 3.5 10.1 4.9 4.7 13.6 5.9 5.9 15 15 15
y10 (1+) 11.1 12.4 5.8 11.3 12.2 5.8 15.8 17.4 8.2 15 15 15
y11 (1+) 10.5 6.1 7.4 10 8.8 6.1 14.5 10.7 9.6 15 15 15
y12 (1+) 11.9 7 8.4 11.2 6.2 7.7 16.3 9.4 11.4 15 15 15
sum 4.7 2.5 3 5.4 2.8 2.7 7.2 3.8 4 15 15 15

Additional Resources and Comments