University at Buffalo - The State University of New York
Skip to Content
Engineering sulfotransferases to modify heparan sulfate. - PubMed - NCBI
Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Chem Biol. 2008 Mar;4(3):200-2. doi: 10.1038/nchembio.66. Epub 2008 Jan 27.

Engineering sulfotransferases to modify heparan sulfate.

Author information

1
Division of Medicinal Chemistry and Natural Products, School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

Abstract

The biosynthesis of heparan sulfate (HS) involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations of these residues altered the substrate specificities. Our results demonstrate the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions.

PMID:
18223645
PMCID:
PMC2676843
DOI:
10.1038/nchembio.66
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center