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Assay Portal | Office of Cancer Clinical Proteomics Research

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP2K6 MEK6,MKK6,PRKMK6,SKK3
Sequence Length (AA) Molecular Weight (Da)
334 37492
Protein Name
Dual specificity mitogen-activated protein kinase kinase 6
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSQSKGKKRN PGLKIPKEAF EQPQTSSTPP RDLDSKACIS IGNQNFEVKA
60 70 80 90 100
DDLEPIMELG RGAYGVVEKM RHVPSGQIMA VKRIRATVNS QEQKRLLMDL
110 120 130 140 150
DISMRTVDCP FTVTFYGALF REGDVWICME LMDTSLDKFY KQVIDKGQTI
160 170 180 190 200
PEDILGKIAV SIVKALEHLH SKLSVIHRDV KPSNVLINAL GQVKMCDFGI
210 220 230 240 250
SGYLVDSVAK TIDAGCKPYM APERINPELN QKGYSVKSDI WSLGITMIEL
260 270 280 290 300
AILRFPYDSW GTPFQQLKQV VEEPSPQLPA DKFSAEFVDF TSQCLKKNSK
310 320 330 334
ERPTYPELMQ HPFFTLHESK GTDVASFVKL ILGD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-820 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K6
Peptide Sequence
DVKPSNVLINALGQVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
179
Peptide End
194
CPTAC ID
CPTAC-820
Peptide Molecular Mass
1,693.9727
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 10.9 7.5 9.6 13.2 8.2 11.5 17.1 11.1 15 15 15 15
y5 (1+) 12.4 8.5 9.1 23.9 7.8 10.9 26.9 11.5 14.2 15 15 15
y6 (1+) 25 14.1 13.6 24.7 14.7 12 35.1 20.4 18.1 15 15 15
y7 (1+) 15.8 11.6 10.1 16.8 16 10.2 23.1 19.8 14.4 15 15 15
y8 (1+) 20.9 11.8 8.2 22.3 10.3 8.2 30.6 15.7 11.6 15 15 15
b8 (1+) 26.2 15.8 17.8 33 16.5 17.2 42.1 22.8 24.8 15 15 15
b7 (1+) 13.8 8.1 6.4 17.2 10.2 5.4 22.1 13 8.4 15 15 15
b6 (1+) 17.3 9.4 8.6 16.4 13 8 23.8 16 11.7 15 15 15
b5 (1+) 26.7 17.8 19.1 26.5 23.4 23.1 37.6 29.4 30 15 15 15
sum 5.5 3.5 4.5 7.5 5.3 5.5 9.3 6.4 7.1 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-821 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP2K6
Peptide Sequence
GTDVASFVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
321
Peptide End
329
CPTAC ID
CPTAC-821
Peptide Molecular Mass
922.4760
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 14.3 12.4 8 16.5 8.7 9.4 21.8 15.1 12.3 15 15 15
y4 (1+) 14.7 7.8 6.4 12.2 8.2 6.3 19.1 11.3 9 15 15 15
y5 (1+) 10.2 4.5 5.9 11.4 5.4 5.1 15.3 7 7.8 15 15 15
y6 (1+) 11.2 6.3 7.8 11.9 6.8 6.8 16.3 9.3 10.3 15 15 15
y7 (1+) 10.2 6.4 4.3 10.5 7.5 4.3 14.6 9.9 6.1 15 15 15
b4 (1+) 17.7 6 11 16.8 8.9 12.1 24.4 10.7 16.4 15 15 15
b3 (1+) 21.4 6.7 10.8 20.2 13.6 11.9 29.4 15.2 16.1 15 15 15
sum 5.3 2.6 2.6 6.4 2.3 2.9 8.3 3.5 3.9 15 15 15

Additional Resources and Comments