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anti-IL4 Receptor antibody (Biotin) | Product No. ABIN2688938

IL4 Receptor antibody (Interleukin 4 Receptor) (Biotin)

Details for Product anti-IL4R Antibody No. ABIN2688938, Supplier: Log in to see
Antigen
  • IL4R
  • il-4ra
  • zgc:113063
  • CD124
  • IL-4RA
  • IL4RA
  • Il4ra
  • IL4
  • Il4r
  • interleukin 4 receptor
  • interleukin 4 receptor, alpha
  • IL4R
  • il4r
  • LOC100220266
  • Il4r
  • Il4ra
Alternatives
anti-Mouse (Murine) IL4 Receptor antibody for ELISA
Reactivity
Mouse (Murine)
128
59
10
Host
Rat
74
64
24
14
1
Clonality (Clone)
Monoclonal ()
Conjugate
This IL4 Receptor antibody is conjugated to Biotin
11
8
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Neutralization (Neut), ELISA
96
57
56
32
29
11
11
7
6
3
3
3
2
2
2
2
2
1
1
Options
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Brand BD Pharmingen™
Immunogen Mouse CTLL-19.4 T Cell Line
Clone MIL4R
Isotype IgG2a, kappa
Characteristics The mIL4R-M1 monoclonal antibody specifically binds to CD124 which is also known as the α subunit of the mouse Interleukin-4 Receptor (IL-4Rα). The mouse IL-4Rα is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and nonhematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc, aka, type I IL-4R) or the IL-13 receptor alpha subunit (IL-4Rα/IL-13Rα, aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R binds and transduces signals from either IL-4 or IL-13. The mIL4R-M1 antibody blocks IL-4 binding to cells and is reported to be a potent inhibitor of IL-4's biological activities. The mIL4R-M1 antibody also recognizes naturally-occurring, soluble truncated forms of IL-4Rα (sIL-4R) that result either from enzymatic cleavage of the cell surface extracellular IL-4Rα domain or from differential mRNAsplicing and secretion by cells. These sIL-4R retain their high-affinity ligand binding domain and appear to either enhance or inhibit IL-4-mediated functions depending on the relative local levels of IL-4 and sIL-4R. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block™ (Cat. No. 553142) to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 (0.125 μg/10^6 cells) followed by streptavidin phycoerythrin (0.015 μg, Cat. No. 554061) and FITC anti-mouse B220 (0.06 μg, Cat. No. 553088) and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells (with the light-scattering characteristics of viable lymphocytes) are shown in the two-color dot plot (right panel). Staining with the biotinylated mIL4R-M1 antibody (right panel) is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody (0.125 μg, Cat. No. 553928) that is shown in the left panel.

BD Pharmingen™ Biotin Rat Anti-Mouse CD124 - Biotin - Clone mIL4R-M1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD124 (IL4R Antibody Abstract)
Pathways JAK-STAT Signaling, Positive Regulation of Immune Effector Process
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product cited in: Hassuneh, Nagarkatti, Nagarkatti: "Evidence for the participation of interleukin-2 (IL-2) and IL-4 in the regulation of autonomous growth and tumorigenesis of transformed cells of lymphoid origin." in: Blood, Vol. 89, Issue 2, pp. 610-20, 1997 (PubMed).

Sempowski, Beckmann, Derdak, Phipps: "Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3606-14, 1994 (PubMed).

Chilton, Fernandez-Botran: "Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5907-17, 1994 (PubMed).

Feldman, Ruhl, Bickel, Finbloom, Pluznik: "Regulation of interleukin-4 receptors on murine myeloid progenitor cells by interleukin-6." in: Blood, Vol. 78, Issue 7, pp. 1678-84, 1991 (PubMed).