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Msp impairs actin uncapping in response to fMLP stimulation.
A) Immunoblotting of partially permeabilized neutrophils was used to quantify release of the actin capping proteins gelsolin (Left) and capZ (right). Cells were partially permeabilized prior to Msp treatment followed by fMLP stimulation, and released protein supernatants were analyzed. Representative immunoblots are shown. B) Graphs of densitometry analysis of immunoblots represent mean ± SEM (n = 3, * P<0.05, ** P<0.01 unpaired t test).