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The Clinical Proteomic Technologies for Cancer | Details for Serpin Peptidase Inhibitor, Clade A, Member 1

Serpin Peptidase Inhibitor, Clade A, Member 1


Background

Catalog Number:

CPTC-SERPINA1-1

Target Antigen:

Serpin Peptidase Inhibitor, Clade A, Member 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

06/13/2017

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-SERPINA1-1 as primary Ab against SERPINA1 (rAg 11019) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-SERPINA1-1 Western blot

Western Blot using CPTC-SERPINA1-1 as primary Ab against SERPINA1 (rAg 11019) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-SERPINA1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-SERPINA1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-SERPINA1-1 NCI 60 Protein Array

Protein Array in which CPTC-SERPINA1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-SERPINA1-1. Titer: 1:4000 Click image to enlarge

CPTC-SERPINA1-1 IHC Tissue

Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-SERPINA1-1. Titer: 1:4000

Background

NCI Identification Number:

11019

Antigen Name:

Serpin Peptidase Inhibitor, Clade A, Member 1

CPTC Name:

CPTC-SERPINA1

Keywords:

AAT; PI; PI1; alpha1AT1; alpha-1-antitrypsin1; A1AT; A1A; Serpin A1; Alpha-1 protease inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase,
antitrypsin), member 1; OTTHUMP00000197154; MGC23330; OTTHUMP00000197150;PRO2275; MGC9222; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; alpha-1-antiproteinase

Function:

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver
disease. Several transcript variants encoding the same protein have been found for this gene.

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for
plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form
inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.

Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not
trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic
destruction by human leukocyte elastase (HLE).

Chromosomal Localization:

14q32.1

Expression System:

E. Coli

NCBI Accession Number:

BC015642.2

Swiss Prot Accession Number:

P01009

DNA Source:

OpenBiosystems - MHS1011-76439

Immunogen:

Recombinant Full Length Protein

Vector Name:

xx

Extinction Coefficient:

19940

Buffers:

PBS without Ca++ and Mg++

Expressed Sequence:

SNAEDPQGDAAQKTDTSHHDQDHPTFNKITPNLAEFAFSLYRQLAHQSNSTNIFFSPVSIATAFAML
SLGTKADTHDEILEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTGNGLFLSEGLKLVDKFL
EDVKKLYHSEAFTVNFGDTEEAKKQINDYVEKGTQGKIVDLVKELDRDTVFALVNYIFFKGKWERPF
EVKDTEEEDFHVDQATTVKVPMMKRLGMFNIQHCKKLSSWVLLMKYLGNATAIFFLPDEGKLQHLEN
ELTHDIITKFLENEDRRSASLHLPKLSITGTYDLKSVLGQLGITKVFSNGADLSGVTEEAPLKLSKA
VHKAVLTIDEKGTEAAGAMFLEAIPMSIPPEVKFNKPFVFLMIEQNTKSPLFMGKVVNPTQK

Native Sequence:

MPSSVSWGILLLAGLCCLVPVSLAEDPQGDAAQKTDTSHHDQDHPTF
NKITPNLAEFAFSLYRQLAHQSNSTNIFFSPVSIATAFAMLSLGTKA
DTHDEILEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTGNG
LFLSEGLKLVDKFLEDVKKLYHSEAFTVNFGDTEEAKKQINDYVEKG
TQGKIVDLVKELDRDTVFALVNYIFFKGKWERPFEVKDTEEEDFHVD
QVTTVKVPMMKRLGMFNIQHCKKLSSWVLLMKYLGNATAIFFLPDEG
KLQHLENELTHDIITKFLENEDRRSASLHLPKLSITGTYDLKSVLGQ
LGITKVFSNGADLSGVTEEAPLKLSKAVHKAVLTIDEKGTEAAGAMF
LEAIPMSIPPEVKFNKPFVFLMIEQNTKSPLFMGKVVNPTQK

Calculated Isoelectric Point:

5.37

Molecular Weight:

44569

Last Updated:

11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-SERPINA1-1. Titer: 1:4000
Click image to enlarge

CPTC-SERPINA1 PAGE

PAGE of Ag 11019 (with molecular weight standards in lane 1)

SOPs

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