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PubMed Central, Figure 2: Nat Cell Biol. 2014 Oct; 16(10): 1004–13. Published online 2014 Sep 21. doi:  10.1038/ncb3040
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
PMC full text:
Nat Cell Biol. Author manuscript; available in PMC 2015 Apr 1.
Published in final edited form as:
Nat Cell Biol. 2014 Oct; 16(10): 1004–13.
Published online 2014 Sep 21. doi:  10.1038/ncb3040

Figure 2

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ΔNp63 deficiency reduces MaSC activity

(a, b) Box plot (a) and western blot (b) of ΔNp63 expression in P4 cells from WT and ΔNp63gfp/+ mice. Boxes in a represents 75th, 50th and 25th percentile of the values. Top and bottom lines represent maximum and minimal data points within 1.5x IQ (inter quarter) range, respectively. P value computed by Mann Whitney U test (n = 5 samples per genotype). (c) Representative mammary outgrowths from WT and ΔNp63gfp/+ mice. (d) Representative FACS profiles of MECs from WT and ΔNp63gfp/+ mouse. (e) Representative mammary outgrowths from f. Each circle below the images represents one mammary gland, with blackened area representing the degree of gland filling with outgrowth. 80–100%, An external file that holds a picture, illustration, etc.
Object name is nihms622121ig1.jpg 30–80%, ◔ 0–30% and no reconstitution. (f) Table showing transplantation of limiting numbers of P4 cells into cleared mammary fat pads from indicated mice (n= number of mammary fat pad injections as indicated in the table). P value was obtained by Pearson’s Chi-squared test using ELDA software. (g) Fold change (FC) of ΔNp63 mRNA level in primary MECs transduced with control and two ΔNp63 shRNA lentiviral constructs (ΔN KD1 and ΔN KD2) (left panel). Fold change of TAp63 mRNA level in control or ΔNp63-KD MECs (right panel). (h) Western blot of ΔNp63 in control and ΔNp63 KD MECs. (i) Fold change of TAp63 mRNA level in primary MECs transduced with control and two TAp63 shRNA lentiviral constructs. TA isoform-specific KD of TAp63 was confirmed by qRT-PCR since TAp63 protein is undetectable in MECs. (j) Quantification of mammosphere formed by control, ΔNp63-KD and TAp63-KD P4 cells (20,000 cells) from WT mice mammary gland. In g, i and j, n = 3 samples; data represents mean ± s.d. *p < 0.05 by Student’s t test. (k) GSEA demonstrating enrichment of MaSC gene signatures in WT versus ΔNp63gfp/+ MECs using MaSC signatures derived from the current study (left panel) or a previously reported MaSC gene signature64 (right panel). Size bar, 2 mm and 4 mm in c and e respectively. Uncropped images of blots are shown in Supplementary Fig. 9.

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